IADR Abstract Archives
Cellular Effects from Metal Brackets after Immersion in Beverages
Objectives: Metal bracket can be release some metal ions in different solutions. The purpose of this study was to investigate the cellular effects of the elutes from conventional and self-ligating (SL) metal brackets after immersion in beverages in vitro.
Methods: There were conventional metal bracket (CB) and SL metal brackets (SLBs) used in the present experiment. The beverages used were black tea, lemon tea and cola. After the metal brackets were immersed for one day, seven days and sixty days, bracket surface morphology was assessed by scanning electron microscopy and energy-dispersive X ray analysis. The metal ions in the eluate were detected by inductively coupled plasma-mass spectrometry. A bone marrow mesenchymal stem cell (BMSC) line was added to the eluate to detect cell viability and genotoxicity.
Results: The results revealed pit corrosion on the surfaces of the CB and SL brackets. The Damon Q showed higher Ni and Cr ion release than the OPK or T1 brackets in all beverages. The T1 showed higher Fe ion release in black tea and cola. BMSC viability decreased in all groups by the 4th day of culture. The lowest cell viability was the Damon Q in lemon tea group (p<0.05). The sub-G1 content of the BMSC culture increased in the black tea and lemon tea conditions (p<0.05) indicating genotoxicity.
Conclusions: Apoptosis was the mechanism of BMSC cell death in all experiment groups. The CB and SLBs can corrode and release metal ions, which may cause unwanted side effects to cells.
Methods: There were conventional metal bracket (CB) and SL metal brackets (SLBs) used in the present experiment. The beverages used were black tea, lemon tea and cola. After the metal brackets were immersed for one day, seven days and sixty days, bracket surface morphology was assessed by scanning electron microscopy and energy-dispersive X ray analysis. The metal ions in the eluate were detected by inductively coupled plasma-mass spectrometry. A bone marrow mesenchymal stem cell (BMSC) line was added to the eluate to detect cell viability and genotoxicity.
Results: The results revealed pit corrosion on the surfaces of the CB and SL brackets. The Damon Q showed higher Ni and Cr ion release than the OPK or T1 brackets in all beverages. The T1 showed higher Fe ion release in black tea and cola. BMSC viability decreased in all groups by the 4th day of culture. The lowest cell viability was the Damon Q in lemon tea group (p<0.05). The sub-G1 content of the BMSC culture increased in the black tea and lemon tea conditions (p<0.05) indicating genotoxicity.
Conclusions: Apoptosis was the mechanism of BMSC cell death in all experiment groups. The CB and SLBs can corrode and release metal ions, which may cause unwanted side effects to cells.