IADR Abstract Archives
Evaluation of Dentin Permeability of Anti-Bacterial and Anti-Inflammatory Drugs
Objectives: Stimuli from oral cavity may affect the dental pulp through exposed dentinal tubules and evoke pulp inflammatory response. Anti-bacterial and anti-inflammatory drugs applied to exposed dentinal tubules and infiltrated through the tubules may give a chance for pulp recovery. Therefore, the purpose of this study is to investigate the dentin permeability of different anti-bacterial and anti-inflammation drugs by using the transwell dentin disc model.
Methods: Thirty-five extracted human third molars without any decays and restorations were used in this study. 0.5mm thickness dentin disks were prepared at coronal plane of the crown without perforation. Two anti-bacterial drugs (Ampicillin sodium and Clindamycin phosphate), two steroid drugs (Betamethasone sodium phosphate and Hydrocortisone sodium succinate), and three NSAID drugs (Piroxicam, Lysine acetylsalicylate, and Diclofenac sodium) were selected. The drug solution was injected into the plastic tube of the transwell dentin disc model and then the filtrate was collected for testing the absorbance by UV-visible spectroscopy. The cumulative release of the drug in 4 hours was recorded.
Results: At the time of the 10th minutes, the cumulative release of all tested drugs were lower than 5%. Even though at the 60thminutes, almost all the detected values were lower than 10%. After four hours of drug solution infiltration, only Ampicillin sodium and Lysine acetylsalicylate were higher than 20%.
Conclusions: Although most of drugs tested in the study showed some potential in infiltrating through dentinal tubules, only Ampicillin sodium and Lysine acetylsalicylate revealed better releasing amount. The dentinal tubule infiltration rate of the anti-bacterial and anti-infiltratory drug solution tested in this study is mostly ineffective. Other vehicles should be developed to enhance the dentin permeability of the drug.
Methods: Thirty-five extracted human third molars without any decays and restorations were used in this study. 0.5mm thickness dentin disks were prepared at coronal plane of the crown without perforation. Two anti-bacterial drugs (Ampicillin sodium and Clindamycin phosphate), two steroid drugs (Betamethasone sodium phosphate and Hydrocortisone sodium succinate), and three NSAID drugs (Piroxicam, Lysine acetylsalicylate, and Diclofenac sodium) were selected. The drug solution was injected into the plastic tube of the transwell dentin disc model and then the filtrate was collected for testing the absorbance by UV-visible spectroscopy. The cumulative release of the drug in 4 hours was recorded.
Results: At the time of the 10th minutes, the cumulative release of all tested drugs were lower than 5%. Even though at the 60thminutes, almost all the detected values were lower than 10%. After four hours of drug solution infiltration, only Ampicillin sodium and Lysine acetylsalicylate were higher than 20%.
Conclusions: Although most of drugs tested in the study showed some potential in infiltrating through dentinal tubules, only Ampicillin sodium and Lysine acetylsalicylate revealed better releasing amount. The dentinal tubule infiltration rate of the anti-bacterial and anti-infiltratory drug solution tested in this study is mostly ineffective. Other vehicles should be developed to enhance the dentin permeability of the drug.