IADR Abstract Archives
Calcium Silicate Cement Contained Strontium for Bone Tissue Engineering
Objectives: The aim of this study was to consider the strontium-modified calcium silicate powder (Sr-CS) that could release biologically effective doses of Si and Sr ions and thus could promote the regeneration of bone defects.
Methods: In this study, three Sr-containing CS materials, Sr5 and Sr10, were prepared. The Sr5 and Sr10 raw materials with a nominal composition of 5% and 10% SrO-doped in CS were prepared by sinter method. The hydrophilicity and hardness were measured. The effects of the ionic product from Sr-CS on osteogenesis differentiation of mesenchymal stem cells, angiogenesis differentiation of human umbilical vein endothelial cells (HUVEC) were examined.
Results: The hydrophilicity of the cement was further significantly improved after doping Sr into CS and the tensile strength up to 3.9 MPa. The Sr-CS exhibit hydroxyapatite sphere formation on materials’ surface was found within 12 h of immersion in SBF. The Sr and Si ions released from Sr-CS promoted cell adhesion, proliferation, and alkaline phosphatase (ALP) activity of hMSCs without addition of the osteogenic reagents. The ionic extract also stimulated HUVEC proliferation and angiogenesis.
Conclusions:
The Sr-contained CS cement is a viable way to stimulate the biological response of hMSCs and HUVEC that were a promising bioactive material for bone tissue-engineering applications.
Methods: In this study, three Sr-containing CS materials, Sr5 and Sr10, were prepared. The Sr5 and Sr10 raw materials with a nominal composition of 5% and 10% SrO-doped in CS were prepared by sinter method. The hydrophilicity and hardness were measured. The effects of the ionic product from Sr-CS on osteogenesis differentiation of mesenchymal stem cells, angiogenesis differentiation of human umbilical vein endothelial cells (HUVEC) were examined.
Results: The hydrophilicity of the cement was further significantly improved after doping Sr into CS and the tensile strength up to 3.9 MPa. The Sr-CS exhibit hydroxyapatite sphere formation on materials’ surface was found within 12 h of immersion in SBF. The Sr and Si ions released from Sr-CS promoted cell adhesion, proliferation, and alkaline phosphatase (ALP) activity of hMSCs without addition of the osteogenic reagents. The ionic extract also stimulated HUVEC proliferation and angiogenesis.
Conclusions:
The Sr-contained CS cement is a viable way to stimulate the biological response of hMSCs and HUVEC that were a promising bioactive material for bone tissue-engineering applications.