IADR Abstract Archives
EDTA Promotes Dentine Integration of Stem Cells Delivered by Hydrogel
Objectives: Ethylenediaminetetraacetic acid (EDTA) has been documented to increase exposure of the growth factors on dentine surface which showed to affect cell proliferation and differentiation in 2D-cell culture. However, there is little information on the fully pulp tissue engineering in a 3D-pulp space. Therefore, this study aims to compare the effects of EDTA-treated and untreated dentine on the proliferation and differentiation of dental pulp stem cells (DPSCs) growing in the fibrin hydrogel scaffolds.
Methods: 1x104cells of DPSCs were seeded in the fibrin hydrogel, which was delivered into 3 conditions; EDTA-treated teeth, untreated teeth and silicone tubes (control). Cell proliferation was investigated by MTT assay while cell differentiation was examined by morphological observation under light microscope at day 0, 14 and 21. One-way ANOVA was used for comparing the cell proliferation among each group whereas the descriptive analysis was obtained for comparing the cell differentiation.
Results: DPSCs were able to proliferate in the 3D-fibrin scaffold in all conditions over 14 and 21 days but there is no statistically significant difference (P>0.05) between EDTA-treated and untreated group. However, histological analysis showed that DPSCs in the EDTA-treated group were densely localized at the dentine interface where they could attach and turn into cells similar to odontoblast-like cells. Furthermore, DPSCs at the center of the fibrin hydrogel showed better cell adhesion and cell-cell interaction in the EDTA-treated group. Meanwhile, the untreated teeth and the control group did not show neither cell-dentine integration nor cell-cell communication.
Conclusions: Fibrin hydrogel can be used to deliver DPSCs for pulp tissue engineering. On top of that, EDTA might enhance the signaling capacity of the dentine during cell transplantation in the root canal by exposing several growth factors, which are necessary for proliferation and differentiation of DPSCs.
Methods: 1x104cells of DPSCs were seeded in the fibrin hydrogel, which was delivered into 3 conditions; EDTA-treated teeth, untreated teeth and silicone tubes (control). Cell proliferation was investigated by MTT assay while cell differentiation was examined by morphological observation under light microscope at day 0, 14 and 21. One-way ANOVA was used for comparing the cell proliferation among each group whereas the descriptive analysis was obtained for comparing the cell differentiation.
Results: DPSCs were able to proliferate in the 3D-fibrin scaffold in all conditions over 14 and 21 days but there is no statistically significant difference (P>0.05) between EDTA-treated and untreated group. However, histological analysis showed that DPSCs in the EDTA-treated group were densely localized at the dentine interface where they could attach and turn into cells similar to odontoblast-like cells. Furthermore, DPSCs at the center of the fibrin hydrogel showed better cell adhesion and cell-cell interaction in the EDTA-treated group. Meanwhile, the untreated teeth and the control group did not show neither cell-dentine integration nor cell-cell communication.
Conclusions: Fibrin hydrogel can be used to deliver DPSCs for pulp tissue engineering. On top of that, EDTA might enhance the signaling capacity of the dentine during cell transplantation in the root canal by exposing several growth factors, which are necessary for proliferation and differentiation of DPSCs.
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