IADR Abstract Archives
Probiotic Lactobacillus-reuteri Consumption Reduced Candida-albicans and Total Bacterial Load in Saliva
Objectives: Probiotic Lactobacillus reuteri is a potential agent to maintain oral health with abilities to produce antimicrobial and antifungal substances. The aim of this study is to determine the effect of L.reuteri probiotic consumption on decreasing Candida-albicans and total number of bacteria in the saliva subjects undergoing fixed orthodontic treatment.
Methods: Saliva (n=10, 18-24 years-old) was collected before and two weeks after consumption of the L. reuteri DSM-17938 probiotic lozenges administered (2x108 CFU/tablet) (BioGaiaÒ ProDentis, Sweden). Plate count method was used to quantify the total number of bacteria in saliva. Saliva samples were cultured into Brain Heart Infusion agar, 37°C, 24h, anaerob condition. C.albicans number were quantified using Real-Time PCR with SYBR-Green using 18S rRNA gene-specific primers. C.albicans ATCC-10231 were cultured in BHI-broth medium at 37°C, aerob condition. After 24-h incubation, the number of colonies on each dilution plate would be used to extrapolate a standard curve. DNA of C.albicans and bacteria in saliva samples were extracted with heat-shock method. Primers were used in this study: 5’-CCCAGTCTTTCACAAGCAGTAAAT-3’ (fwd) and 5’-GTAAATGAGTCA TCAACAGAAGCC-3’ (rvs). This study has obtained the permission from ethics committee with number: 122/S3/KEPK/FKG/1/2018. Data were statistically analyzed using paired T-test to reveal significant differences of bacterial number before and after probiotic consumption. Level of significance was set at p<0.05
Results: Results showed a significant reduction of total bacterial number in all saliva samples before (3.30±0.31 LogCFU/mL) and 2 weeks after probiotic consumption (2.88±0.24 LogCFU/mL). Total number of C. albicans in saliva samples also significantly decreased before (3.21±2.39 LogCFU/mL) and after probiotic consumption (1.18±1.03 LogCFU/mL). Both statistical test results showed significant difference before and 2 weeks after probiotic consumption (p<0.05).
Conclusions: Probiotic L.reuteri consumption may reduce C.albicans and total bacterial load in saliva of subjects undergoing fixed orthodontic treatment potentially preventing oral diseases. Further studies are needed to confirmed this result.
Methods: Saliva (n=10, 18-24 years-old) was collected before and two weeks after consumption of the L. reuteri DSM-17938 probiotic lozenges administered (2x108 CFU/tablet) (BioGaiaÒ ProDentis, Sweden). Plate count method was used to quantify the total number of bacteria in saliva. Saliva samples were cultured into Brain Heart Infusion agar, 37°C, 24h, anaerob condition. C.albicans number were quantified using Real-Time PCR with SYBR-Green using 18S rRNA gene-specific primers. C.albicans ATCC-10231 were cultured in BHI-broth medium at 37°C, aerob condition. After 24-h incubation, the number of colonies on each dilution plate would be used to extrapolate a standard curve. DNA of C.albicans and bacteria in saliva samples were extracted with heat-shock method. Primers were used in this study: 5’-CCCAGTCTTTCACAAGCAGTAAAT-3’ (fwd) and 5’-GTAAATGAGTCA TCAACAGAAGCC-3’ (rvs). This study has obtained the permission from ethics committee with number: 122/S3/KEPK/FKG/1/2018. Data were statistically analyzed using paired T-test to reveal significant differences of bacterial number before and after probiotic consumption. Level of significance was set at p<0.05
Results: Results showed a significant reduction of total bacterial number in all saliva samples before (3.30±0.31 LogCFU/mL) and 2 weeks after probiotic consumption (2.88±0.24 LogCFU/mL). Total number of C. albicans in saliva samples also significantly decreased before (3.21±2.39 LogCFU/mL) and after probiotic consumption (1.18±1.03 LogCFU/mL). Both statistical test results showed significant difference before and 2 weeks after probiotic consumption (p<0.05).
Conclusions: Probiotic L.reuteri consumption may reduce C.albicans and total bacterial load in saliva of subjects undergoing fixed orthodontic treatment potentially preventing oral diseases. Further studies are needed to confirmed this result.