IADR Abstract Archives
PGE2 Induces BMP9 Expression via EP2 Receptor in PDL Cells
Objectives: Masticatory force is critical for periodontal tissue and alveolar bone remolding. PGE2 is one of several molecules released in response to the mechanical force. Bone morphogenetic proteins (BMP) 9 have high potential in promoting osteoblastic differentiation of MSCs. Since periodontal ligament (PDL) cells have osteogenic differentiation capability, the present study aimed to investigate the effect of PGE2 on the expression of BMP9 and its regulatory pathway in human PDL cells.
Methods: The primary culture of human PDL cells was treated with various doses of PGE2. The non-toxic doses of PGE2 were determined by MTT assay. Cells were harvested for RNA which subsequently analyzed for BMP9 expression by qPCR. PGE2 receptor antagonists were used to identify the functional receptor in the regulatory pathway which analyzed by the expression level of BMP9.
Results: The study demonstrated that 10 uM PGE2 significantly increased BMP9 expression in human PDL cells by 48 hours. Addition of PGE2 receptor EP2 antagonist demonstrated significant decrease of BMP9 expression induced by PGE2, while no change of the expression was found with EP4 receptor antagonist.
Conclusions: Our finding demonstrates that PGE2 induces BMP9 expressions in human PDL cells via EP2 receptor. Further studies are required to clarify an intracellular signaling pathway.
Methods: The primary culture of human PDL cells was treated with various doses of PGE2. The non-toxic doses of PGE2 were determined by MTT assay. Cells were harvested for RNA which subsequently analyzed for BMP9 expression by qPCR. PGE2 receptor antagonists were used to identify the functional receptor in the regulatory pathway which analyzed by the expression level of BMP9.
Results: The study demonstrated that 10 uM PGE2 significantly increased BMP9 expression in human PDL cells by 48 hours. Addition of PGE2 receptor EP2 antagonist demonstrated significant decrease of BMP9 expression induced by PGE2, while no change of the expression was found with EP4 receptor antagonist.
Conclusions: Our finding demonstrates that PGE2 induces BMP9 expressions in human PDL cells via EP2 receptor. Further studies are required to clarify an intracellular signaling pathway.