IADR Abstract Archives
Prostacyclin Promotes Human Dental Pulp Cells Migration Via Matrix Metalloproteinase-9-Related Pathway
Objectives: Dental pulp healing is the crucial event for dental pulp regeneration. This study investigated the role of prostacyclin (PGI2) on promoting human dental pulp cells (HDPCs) migration.
Methods: The wound scratch assay was performed on HDPCs. The HDPCs were treated with PGI2 for 24 and 72 h. The qPCR gene analysis and ELISA were performed. The inhibitor of PGI2 (IP) receptor and protein kinase A (PKA) signaling pathway was performed by the treatment of IP antagonist/PKA inhibitor.
Results: The closure of a mechanical scratch in HDPCs in cell culture was accelerated and reduced the wounded area at 72 h upon the treatment of iloprost. The increase of MMP-9 mRNA and protein expression in hDPCs was observed and these effects were inhibited by PKA-inhibitor. Upon the activation of forskolin, the MMP-9 expression was upregulated.
Conclusions: PGI2 accelerated wound closure in the scratch test assay in HDPCs. The expression of MMP-9 is increased by iloprost by the IP-PKA pathway. These observations suggested that PGI2 can stimulate wound healing through the enhanced production of MMP-9. The treatment of PGI2 might be another promising biomolecule in dental pulp regenerative treatments.
Methods: The wound scratch assay was performed on HDPCs. The HDPCs were treated with PGI2 for 24 and 72 h. The qPCR gene analysis and ELISA were performed. The inhibitor of PGI2 (IP) receptor and protein kinase A (PKA) signaling pathway was performed by the treatment of IP antagonist/PKA inhibitor.
Results: The closure of a mechanical scratch in HDPCs in cell culture was accelerated and reduced the wounded area at 72 h upon the treatment of iloprost. The increase of MMP-9 mRNA and protein expression in hDPCs was observed and these effects were inhibited by PKA-inhibitor. Upon the activation of forskolin, the MMP-9 expression was upregulated.
Conclusions: PGI2 accelerated wound closure in the scratch test assay in HDPCs. The expression of MMP-9 is increased by iloprost by the IP-PKA pathway. These observations suggested that PGI2 can stimulate wound healing through the enhanced production of MMP-9. The treatment of PGI2 might be another promising biomolecule in dental pulp regenerative treatments.