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The Effectiveness Test of Avocado Seed Extract (Persea americana Mill) Against Growth of Candida albicans and Streptococcus mutans

Objectives: To study the effect of avocados seed extract to Candida Albicans dan Streptococcus mutans
Methods: Laboratory experimental study with post test only control group design. Avocado seeds powder was macerated with 96% ethanol, and tested using diffusion method with 6 concentrations groups as follows; 2%, 4%, 6%, 8%, 10% and positive control (nystatin and ampicillin). Each group consist of 4 samples. The inhibition effect analyzed by measuring clear zone around the paper disc. Data were analyzed by Kruskall Wallis Test and Mann-Whitney for Candida Albicans and One-way Anova Test for Streptococcus mutans
Results: Inhibition zone diameter for C. albicans with avocados seed extract result are as follow 2% (6.32mm); 4% (6.79mm); 6% (7.43mm); 8% (8.30mm); 10% (8.92 mm), and positive control (19.49mm). Inhibition zone diameter for S. mutans in avocados seed extract result are as follow 2% (11.51 mm); 4% (12.39mm); 6% (13.61mm); 8% (13.53mm); 10% (15.02 mm), and for positif control (7.89 mm). The higher the avocado seed extract concentration the greater the diameter of the clear zone formed.
Conclusions: In this study, avocados seed extract (Persea americana Mill) inhibits the growth of Candida Albicans and Streptococcus mutans significantly in concentration 10%. It considered to be bactericidal to both microorganism too.
Division: South East Asia Division Meeting
Meeting: 2018 South East Asia Division Meeting (Da Nang, Vietnam)
Location: Da Nang, Vietnam
Year: 2018
Final Presentation ID: 0182
Abstract Category|Abstract Category(s): Prosthodontics Research
Authors
  • Baso, Maharani  ( Hasanuddin University , Maros , South Sulawesi , Indonesia )
    • Financial Interest Disclosure: NONE
    SESSION INFORMATION
    Poster Session
    Poster Session 4-Microbiology and Pharmacology
    Friday, 09/14/2018 , 11:15AM - 12:30PM
    TABLES
    Table 1. Comparison of mean values of inhibition zone between treatment groups against C.albicans
    Concentration2%4%6%8%10%Control +
    Nistatin
    2% 0.0200.0210.0210.0210.021
    4%0.020 0.0200.0200.0200.020
    6%0.0210.020 0.1490.0830.021
    8%0.0210.0200.149 0.1490.021
    10%0.0210.0200.0830.149 0.021
    Control +
    Nistatin    		0.0210.0200.0210.0210.021 

    Table 2. The mean value of standard deviation of avocado seed extract concentration on S.mutans
    ConcentrationMean ± SDP value
    2%11.5100 ± 0.377450.000
    4%12.3925 ± 1.497270.000
    6%13.6150 ± 0.892360.000
    8%13.5350 ± 1.904140.000
    10%15.0275 ± 0.975790.000
    Paired sampel t-test p<0.05; significans
    Table 3. Comparison of mean values of inhibition zone between treatment groups against S.mutans
    Concentration (i)Concentration (j)Mean
    Difference (i-j)    		P value
    2%4%
    6%
    8%
    10%    		-0.88250
    -2.10500
    -2.02500
    -3.51750    		0.296
    0.19
    0.24
    0.00
    4%2%
    6%
    8%
    10%    		0.88250
    -1.22250
    -1.14250
    -2.63500    		0.296
    0.154
    0.181
    0.005
    6%2%
    4%
    8%
    10%    		2.10500
    1.22250
    0.08000
    -1.41250    		0.923
    0.154
    0.923
    0.102
    8%2%
    4%
    6%
    10%    		2.02500
    1.14250
    -0.8000
    -1.49250    		0.24
    0.181
    0.923
    0.102
    10%2%
    4%
    6%
    8%    		3.51750
    2.63500
    1.41250
    1.49250    		0.000
    0.005
    0.102
    0.086
    Paired sampel t-test p<0.05, significans
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