IADR Abstract Archives
The Absorbance Determination of Biofilm Formations by Candida albicans that Induced with Lactosa, Glucosa, Iron and Protein (Soy)
Objectives: Determine the absorbance thickness of biofilm formation from Candida albicans induced by various materials : Lactose, Glucose, Iron and Protein (Soy).
Methods: Growing Candida albicans (C.a) on Saboraud Dextrose Agar (DSA) with 4 treatments. Treatment A: C.a induced by 5% Lactose; Treatment B: C.a with 5% glucose induction; Treatment C: C.a with Iron (FeCl2 10%) induction and Treatment D : is C.a with Soy Protein induction. Whereas E is the growth control of C.a without induction. Each treatment is 6x replicated, then stained with Crystal Violet. Test using microplate assay.
Results: Average OD reads with a wavelength of 492 nm, for Treatment A = 0.279; B = 0.197; C = 0.177; D = 0.297 and E = 0.053. Biofilm C.a induced by Protein and Lactose materials has a much thicker absorbance than the C.a-induced biofilm with Glucose and Iron.
Conclusions: The thickness of absorbance of biofilm formation of C.a is influenced by growth demand factor of C.a.
Methods: Growing Candida albicans (C.a) on Saboraud Dextrose Agar (DSA) with 4 treatments. Treatment A: C.a induced by 5% Lactose; Treatment B: C.a with 5% glucose induction; Treatment C: C.a with Iron (FeCl2 10%) induction and Treatment D : is C.a with Soy Protein induction. Whereas E is the growth control of C.a without induction. Each treatment is 6x replicated, then stained with Crystal Violet. Test using microplate assay.
Results: Average OD reads with a wavelength of 492 nm, for Treatment A = 0.279; B = 0.197; C = 0.177; D = 0.297 and E = 0.053. Biofilm C.a induced by Protein and Lactose materials has a much thicker absorbance than the C.a-induced biofilm with Glucose and Iron.
Conclusions: The thickness of absorbance of biofilm formation of C.a is influenced by growth demand factor of C.a.