Suitability of MDA, 8-OHdG and Wildtype p53 as Genotoxic Biomarkers in Metal (Co, Ni dan Cr) Exposed Dental Technicians:A Cross-Sectional study

Objectives: This study intended to investigate whether the Dental Technician working in area polluted by Co, Ni and Cr, could contribute to the high incidence of cancer exposured at the occupational.
Methods: A cross-sectional study was conducted among dental technicians after ethical clearance. Occupational exposure to metal was assessed by measuring the level of Co, Ni, Cr and control by AAS, MDA is used as biological biomarker of lipid peroxidation that described the degree of oxidative stress were measure by TBARS test, 8-hydroxy-2-deoxyguanosine (8-OHdG) and p53wildtype were measured each with an Elisa check kit and analyzed saliva with indirect ELISA as a biological response to various forms of stress.
Results: Comparative statistical analysis between Co, Ni, and Cr levels in the exposed group with the control group, MDA, 8-OHdG, and Wildtype p53 in blood serum of dental technicians all showed significant differences (p <0.05). A significant correlation was shown between 8 OHdG with Cr: (p = 0.024 r = 0.355), with Co: (P = 0.034, r = 0.337), with p53: (p = 0.001 r = -0.103). and MDA with Co: (p = 0,033, r = 0,337).
Conclusions: MDA, 8-OHdG and p53 can be used as genotoxic biomarkers in the metal exposed group, all showed significant differences (p <0.05) compare to metal levels, that they can accurately reflect the degree of genetic damage.