IADR Abstract Archives
In vitro Evaluation of the Bactericidal Effect and Fibroblast Attachment on Contaminated Rough Titanium Surfaces Treated with Er: YAG Laser
Objectives: Studies have reported a high prevalence of peri-implantitis with radiographic marginal bone loss. Evidence to date indicates that no available treatments result in total resolution of established peri-implantitis. The aim of this study was to investigate the bactericidal effect and fibroblast attachment on contaminated titanium surface treated with erbium-doped yttrium aluminium garnet (Er:YAG) laser.
Methods: Grade IV titanium discs were incubated with a suspension of P. gingivalis (ATCC® 33277TM) in brain heart infusion (BHI) broth. Six groups (n=6) with a total of 36 titanium discs were prepared. Group 1 was the negative control. Group 2 to 6, titanium discs were incubated with P. gingivalis biofilm. Group 3, 0.12 % Chlorhexidine was used for irrigation. Group 4, curette was used for debridement. Group 5, Er:YAG laser irradiation was performed at pulse energy 80 mJ and frequency 25 pulse per second for debridement. Group 6, Curette debridement was followed by Er:YAG laser irradiation. After various treatments, surface roughness (Ra) and hydrophilicity were measured. The residual bacterial was examined by biomass quantification, scanning electron microscope and confocal laser scanning microscope. Then, the adhesion of fibroblast cell was quantified by fluorescent microscope.
Results: Chlorhexidine irrigation or curette debridement alone couldn’t remove bacteria efficiently. Although Er:YAG Laser combined with curette debridement was the most effective therapy in removing bacterial remnants, the adhesion of fibroblast was not as high with clean titanium discs.
Conclusions: Er:YAG laser would be a promising adjuvant therapy to treat peri-implantitis by removing bacterial remnants without altering titanium surface. Further studies are required to evaluate the potential of osteoblast adhesion after Er:YAG laser treatment.
Methods: Grade IV titanium discs were incubated with a suspension of P. gingivalis (ATCC® 33277TM) in brain heart infusion (BHI) broth. Six groups (n=6) with a total of 36 titanium discs were prepared. Group 1 was the negative control. Group 2 to 6, titanium discs were incubated with P. gingivalis biofilm. Group 3, 0.12 % Chlorhexidine was used for irrigation. Group 4, curette was used for debridement. Group 5, Er:YAG laser irradiation was performed at pulse energy 80 mJ and frequency 25 pulse per second for debridement. Group 6, Curette debridement was followed by Er:YAG laser irradiation. After various treatments, surface roughness (Ra) and hydrophilicity were measured. The residual bacterial was examined by biomass quantification, scanning electron microscope and confocal laser scanning microscope. Then, the adhesion of fibroblast cell was quantified by fluorescent microscope.
Results: Chlorhexidine irrigation or curette debridement alone couldn’t remove bacteria efficiently. Although Er:YAG Laser combined with curette debridement was the most effective therapy in removing bacterial remnants, the adhesion of fibroblast was not as high with clean titanium discs.
Conclusions: Er:YAG laser would be a promising adjuvant therapy to treat peri-implantitis by removing bacterial remnants without altering titanium surface. Further studies are required to evaluate the potential of osteoblast adhesion after Er:YAG laser treatment.