IADR Abstract Archives
Nano-calcium Carbonate from Cuttlefish (Sepia officinalis) Bone for Enamel Remineralization
Objectives: Dental caries is caused by the action of bacteria producing acid. Its earliest sign is a presence of chalky white spot on the enamel surface known as incipient caries. Remineralization is the process wherein the lost minerals are added back to the tooth surface. This study aimed to determine the effectiveness of Nano-calcium carbonate from Cuttlefish (Sepia officinalis) bone as an alternative remineralizing agent to Casein phosphopeptide-amorphous calcium phosphate (CPP-ACP).
Methods: Scanning Electron Microscope (SEM) and Fourier Transform Infrared Spectroscopy (FTIR) Analysis confirmed the presence of Nano-calcium carbonate from Cuttlebone. With single-blinded randomized in vitro design, fifteen bovine teeth were equally assigned into three treatment groups, Nano-CaCO3 from cuttlebone (experimental), CPP-ACP (positive control) and untreated (negative control) groups. All groups were submerged into a demineralizing solution for two days to create incipient caries-like lesions. For remineralization process of the experimental and positive control group, Nano-calcium carbonate and CPP-ACP were applied respectively for 30 minutes daily, for 7 days. All groups were submerged in artificial saliva to mimic the pH of oral cavity. The statistical tool that are used were Paired t-test, and One Way ANOVA (Analysis of Variance).
Results: SEM-EDX Analysis showed an improved surface morphology and increased calcium and phosphorus levels for the experimental and positive group, which suggests that enamel remineralization took place. T-test and ANOVA results revealed that statistically, there is no significant difference between the experimental and positive group in terms of mineral atomic and weight concentration of calcium and phosphorus levels because of their p-value greater than 0.05. Thus, enamel treated with Nano-calcium Carbonate from cuttlefish bone is comparable to that of the enamel treated with CPP-ACP in terms of enamel remineralization.
Conclusions: Nano-Calcium carbonate from Cuttlefish (Sepia officinalis) bone has a potential to be a tooth remineralizing agent to prevent the progression of incipient caries lesion.
Methods: Scanning Electron Microscope (SEM) and Fourier Transform Infrared Spectroscopy (FTIR) Analysis confirmed the presence of Nano-calcium carbonate from Cuttlebone. With single-blinded randomized in vitro design, fifteen bovine teeth were equally assigned into three treatment groups, Nano-CaCO3 from cuttlebone (experimental), CPP-ACP (positive control) and untreated (negative control) groups. All groups were submerged into a demineralizing solution for two days to create incipient caries-like lesions. For remineralization process of the experimental and positive control group, Nano-calcium carbonate and CPP-ACP were applied respectively for 30 minutes daily, for 7 days. All groups were submerged in artificial saliva to mimic the pH of oral cavity. The statistical tool that are used were Paired t-test, and One Way ANOVA (Analysis of Variance).
Results: SEM-EDX Analysis showed an improved surface morphology and increased calcium and phosphorus levels for the experimental and positive group, which suggests that enamel remineralization took place. T-test and ANOVA results revealed that statistically, there is no significant difference between the experimental and positive group in terms of mineral atomic and weight concentration of calcium and phosphorus levels because of their p-value greater than 0.05. Thus, enamel treated with Nano-calcium Carbonate from cuttlefish bone is comparable to that of the enamel treated with CPP-ACP in terms of enamel remineralization.
Conclusions: Nano-Calcium carbonate from Cuttlefish (Sepia officinalis) bone has a potential to be a tooth remineralizing agent to prevent the progression of incipient caries lesion.