IADR Abstract Archives
Cytotoxicity of Alstonia scholaris (L.) R.Br. Leaves Extract On Human Dental Pulp Cells
Objectives: Alstonia scholaris (L.) R.Br. are known to have potential in medicine. Its leaves have been used traditionally as remedies in a wide spectrum of biological activities, including anti-inflammatory, analgesic anti-cancer and anti-bacteria. In dentistry, A. scholaris leaves extract has been investigated and suggested as a potential remedy for dental caries. However, to our knowledge the cytotoxity study of A. scholaris leaves extract on dental pulp has not been reported yet. Therefore current study was conducted to investigate the cytotoxicity of A. Scholaris leaves extract on dental pulp.
Methods: A. Scholaris leaves were collected, maserated and extracted with methanol. Meanwhile, human dental pulps were obtained from freshly extracted partial impacted third molar teeth. Pulps were digested with collagenase type I and cultured in DMEM supplemented with FBS and antibiotic-anti-mycotic agent. A. scholaris extract was applied to human dental pulp cell in various concentrations (5000, 1000, 500, 50, 10 µg/mL) for 24 hours. MTT assay was performed to quantify viable human dental pulp cells.
Results: Although high concentration of A. scholaris extract was applied, mostly viable human dental pulp cells were observed. Meanwhile 0.001% H2O2 induced cytotoxicity of human dental pulp cells. The inhibition concentration (IC)50 of A. scholaris extract was 6,345 µg/mL. Interestingly, in comparison with untreated group, A. scholaris extract in low concentration could induce growth of human dental pulp cells.
Conclusions: Methanol leaves extract of A. scholaris did not induced cytotoxicity in human dental pulp cell. In contrary, the extract in low concentration might induce the growth of human dental pulp cells. Further research should be explored to confirm the growth induction activity of the extract.
Methods: A. Scholaris leaves were collected, maserated and extracted with methanol. Meanwhile, human dental pulps were obtained from freshly extracted partial impacted third molar teeth. Pulps were digested with collagenase type I and cultured in DMEM supplemented with FBS and antibiotic-anti-mycotic agent. A. scholaris extract was applied to human dental pulp cell in various concentrations (5000, 1000, 500, 50, 10 µg/mL) for 24 hours. MTT assay was performed to quantify viable human dental pulp cells.
Results: Although high concentration of A. scholaris extract was applied, mostly viable human dental pulp cells were observed. Meanwhile 0.001% H2O2 induced cytotoxicity of human dental pulp cells. The inhibition concentration (IC)50 of A. scholaris extract was 6,345 µg/mL. Interestingly, in comparison with untreated group, A. scholaris extract in low concentration could induce growth of human dental pulp cells.
Conclusions: Methanol leaves extract of A. scholaris did not induced cytotoxicity in human dental pulp cell. In contrary, the extract in low concentration might induce the growth of human dental pulp cells. Further research should be explored to confirm the growth induction activity of the extract.