IADR Abstract Archives
A novel model of substitute for human dentin barrier tests
Objectives: The dentin barrier test, which mimics the diffusion of components from restorative materials via dentin in vivo, is widely used in vitro cytotoxic testing. Due to the large variation of dentin and difficult collection of human teeth, some researchers have tried to find substitutes for human dentin. In the experiments, we used Sawbones®, a commercialized biomechanical material, to compare with human dentin in evaluation of eluted HEMA concentration.
Methods: Extracted human molars were collected. Human dentin and Sawbone® discs (Solid Rigid Polyurethane Foam 40 pcf Density) were prepared in two thickness, 0.5 mm and 1.5 mm. Two kinds of dentin bonding systems, Single Bond Universal Adhesive (SBU, 3M ESPE) as self-etch system and AdperTM Single Bond 2 (ASB, 3M ESPE) as total-etch system, were used and photo-cured according to manufacturer instruction. The leached-out concentration of HEMA through the dentin and Sawbone® (after 1hr, 4hr, 8hr, 12hr, 24hr, 48hr, 72hr) were quantified by high performance liquid chromatography (HPLC). Statistical analyses were performed using the T-test.
Results: The result of HPLC showed that:
1. HEMA concentration (after 72hr) was higher in SBU in both thickness of dentin (p<0.05).
2. With ASB in both thickness, HEMA concentration (after 72hr) was higher in dentin than in Sawbone® (p<0.05).
3. There was positive correlation of accumulating leached-out HEMA concentration on dentin barrier group and Sawbone® group.
Conclusions: 1. The leached-out concentration of HEMA was higher in SBU groups.
2. The concentration of HEMA was higher in 0.5mm thickness with same barrier material and same bonding agent.
3. Sawbone® is a stable and predictable substitute for human dentin barrier tests.
Methods: Extracted human molars were collected. Human dentin and Sawbone® discs (Solid Rigid Polyurethane Foam 40 pcf Density) were prepared in two thickness, 0.5 mm and 1.5 mm. Two kinds of dentin bonding systems, Single Bond Universal Adhesive (SBU, 3M ESPE) as self-etch system and AdperTM Single Bond 2 (ASB, 3M ESPE) as total-etch system, were used and photo-cured according to manufacturer instruction. The leached-out concentration of HEMA through the dentin and Sawbone® (after 1hr, 4hr, 8hr, 12hr, 24hr, 48hr, 72hr) were quantified by high performance liquid chromatography (HPLC). Statistical analyses were performed using the T-test.
Results: The result of HPLC showed that:
1. HEMA concentration (after 72hr) was higher in SBU in both thickness of dentin (p<0.05).
2. With ASB in both thickness, HEMA concentration (after 72hr) was higher in dentin than in Sawbone® (p<0.05).
3. There was positive correlation of accumulating leached-out HEMA concentration on dentin barrier group and Sawbone® group.
Conclusions: 1. The leached-out concentration of HEMA was higher in SBU groups.
2. The concentration of HEMA was higher in 0.5mm thickness with same barrier material and same bonding agent.
3. Sawbone® is a stable and predictable substitute for human dentin barrier tests.
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