IADR Abstract Archives
The promotion of osteointegration using calcium silicate/chitosan composites on 3D-printed titanium scaffolds
Objectives: The calcium silicate/chitosan (CS/CH) composites in 3D-printed titanium scaffolds is a promising approach to produce biomaterials with better osseointegration capacity. In addition, the natural bone has an ordered composition of organic fibers with dispersion of inorganic apatite among them.
Methods: In this study, Ti scaffolds were fabricated with 3D printing and coated with CS/CH mixer. The microstructure, chemical component, and contact angle of CS/CH-Ti composites were analyzed by scanning electron microscopy, X-ray diffraction, and Fourier transform infrared spectroscopy. In vitro, various CS/CH-Ti increased the formation of hydroxyapatite on the specimens’ surface when soaked in cell cultured medium.
Results: During culture, several biological characteristics of the human mesenchymal stem cells (hMSCs) cultured on CS/CH-Ti groups were promoted as compared to those on pure Ti scaffold. Increased secretion levels of Collagen I and fibronectin were observed in calcium silicate-powder content.
Conclusions: Furthermore, with comparison to Ti scaffold without CS/CH, CS10 and CS15 mats markedly enhanced the proliferation of hMSCs and their osteogenesis properties, which was characterized by osteogenic-related gene expression. These results clearly demonstrated that the biodegradable and electroactive CS/CH-Ti scaffolds are an ideal and suitable candidate for bone tissue engineering.
Methods: In this study, Ti scaffolds were fabricated with 3D printing and coated with CS/CH mixer. The microstructure, chemical component, and contact angle of CS/CH-Ti composites were analyzed by scanning electron microscopy, X-ray diffraction, and Fourier transform infrared spectroscopy. In vitro, various CS/CH-Ti increased the formation of hydroxyapatite on the specimens’ surface when soaked in cell cultured medium.
Results: During culture, several biological characteristics of the human mesenchymal stem cells (hMSCs) cultured on CS/CH-Ti groups were promoted as compared to those on pure Ti scaffold. Increased secretion levels of Collagen I and fibronectin were observed in calcium silicate-powder content.
Conclusions: Furthermore, with comparison to Ti scaffold without CS/CH, CS10 and CS15 mats markedly enhanced the proliferation of hMSCs and their osteogenesis properties, which was characterized by osteogenic-related gene expression. These results clearly demonstrated that the biodegradable and electroactive CS/CH-Ti scaffolds are an ideal and suitable candidate for bone tissue engineering.