IADR Abstract Archives
Development of collagen-chitosan hydrogels for periodontal bony defect regeneration
Objectives: Periodontitis often causes irregular alveolar bony defects that are difficult to compact particulate graft materials, resulting in poor treatment prognosis. The major purpose of this study was to develop an injectable thermosensitive collagen-chitosan composite hydrogel which could be applied to bony defects and polymerized in situ. This study was further investigated the characteristics of hydrogel which was composed of different ratios of type I collagen and chitosan, to obtain the optimum conditions of hydrogel for bony defect regeneration.
Methods: Collagen and chitosan were mixed in different ratios that collagen to chitosan were 10:0(Col), 9:1(Ch-1), 8:2(Ch-2) and 5:5(Ch-5). Fourier transform infrared spectroscopy (FTIR) was used to analyze the hydrogel composition. Various physical properties of hydrogels including mechanical properties, resistance to enzymatic degradation and swelling property were investigated. The morphology of hydrogels was observed using a scanning electron microscopy (SEM). The cytotoxicity was analyzed according to the method of ISO10993-5. In addition, the osteoblastic proliferation was examined with the alamarBlue reagents. Furthermore, osteoblastic mineralization was analyzed with Von Kossa staining.
Results: The FTIR results showed no significant difference of hydrogels compositions compared to the compositions of pure collagen and pure chitosan. The SEM images showed nanoscale collagen fibrils interspersed with chitosan matrix. For mechanical properties, the hydrogel containing chitosan revealed higher young’s modulus than the pure collagen one. In enzymatic degradation, Ch-5 showed higher enzymatic resistance than the other counter parts. Swelling ratio results showed that the scaffolds could bind more water as the chitosan composition increased. In cytotoxicity test, no significant toxicity was found among groups. However, Col and Ch-1 showed more osteoblastic proliferation than Ch-2 and Ch-5 counter groups. For mineralization, Ch-5 showed the lowest mineralization properties among groups.
Conclusions: Col and Ch-1 hydrogels showed higher osteoblastic proliferation and favorable mineralization properties, but Ch-1 revealed higher young’s modulus than the Col group.
Methods: Collagen and chitosan were mixed in different ratios that collagen to chitosan were 10:0(Col), 9:1(Ch-1), 8:2(Ch-2) and 5:5(Ch-5). Fourier transform infrared spectroscopy (FTIR) was used to analyze the hydrogel composition. Various physical properties of hydrogels including mechanical properties, resistance to enzymatic degradation and swelling property were investigated. The morphology of hydrogels was observed using a scanning electron microscopy (SEM). The cytotoxicity was analyzed according to the method of ISO10993-5. In addition, the osteoblastic proliferation was examined with the alamarBlue reagents. Furthermore, osteoblastic mineralization was analyzed with Von Kossa staining.
Results: The FTIR results showed no significant difference of hydrogels compositions compared to the compositions of pure collagen and pure chitosan. The SEM images showed nanoscale collagen fibrils interspersed with chitosan matrix. For mechanical properties, the hydrogel containing chitosan revealed higher young’s modulus than the pure collagen one. In enzymatic degradation, Ch-5 showed higher enzymatic resistance than the other counter parts. Swelling ratio results showed that the scaffolds could bind more water as the chitosan composition increased. In cytotoxicity test, no significant toxicity was found among groups. However, Col and Ch-1 showed more osteoblastic proliferation than Ch-2 and Ch-5 counter groups. For mineralization, Ch-5 showed the lowest mineralization properties among groups.
Conclusions: Col and Ch-1 hydrogels showed higher osteoblastic proliferation and favorable mineralization properties, but Ch-1 revealed higher young’s modulus than the Col group.