IADR Abstract Archives
The effects of blood cockle’s shell and golden sea cucumber on osteoblast-osteoclast in vivo
Objectives: The complication of tooth extraction, periodontal disease, pathological conditions can followed by the alveolar bone loss. Calcium carbonate derived from blood cockle shells has an osteoconductive properties. On the other hand, golden sea cucumber contains hyaluronic acid can stimulate the healing process by decreasing the pro-inflamatory cytokines. We have developed the sea cucumber and calcium carbonate from blood cockle shells for biomedical application. This study aimed to determined the effects of combination blood cockle shell and sea cucumber on osteoblast and osteoclast’s number in vivo.
Methods:
25 male wistar rats were divided into 5 groups: Group 1= sham; Group 2= blood cockle’s shell; Group 3= blood cockle’s shell and sea cucumber 0.4%; Group 4= blood cockle’s shell and sea cucumber 0.8%; Group 5= blood cockle’s shell and sea cucumber 1.6%. The graft material were implanted into rat femur for 14 days. Thereafter, histological analysis were perfomed and the number of osteoclast and osteoblast were evaluated. The statistical anaysis done by Anova One-Way, LSD, p<0.05.
Results: The average number of osteoblast showed in Group 1 (9.7±3.6), Group 2 (20.7±0.5), Group 3 (32.46±0.557), Group 4 (40.73±0.894) and Group 5 (49.5±1.178). On the other hand, the average number of osteoclast showed in Group 1 (12.5±2.8), Group 2 (4.2±2.1), Group 3 (3.8±0.8), Group 4 (2.8±1.3) and Group 5 (2.0±0.9). In the comparison between the groups, there were significant differences for the number of osteoblast and osteoclast.
Conclusions:
It was revealed from our study that the combination graft material that contain with blood cockle’s shell and sea cucumber 1.6% has a good effect on the number of osteoblast and osteoclast in vivo.
Methods:
25 male wistar rats were divided into 5 groups: Group 1= sham; Group 2= blood cockle’s shell; Group 3= blood cockle’s shell and sea cucumber 0.4%; Group 4= blood cockle’s shell and sea cucumber 0.8%; Group 5= blood cockle’s shell and sea cucumber 1.6%. The graft material were implanted into rat femur for 14 days. Thereafter, histological analysis were perfomed and the number of osteoclast and osteoblast were evaluated. The statistical anaysis done by Anova One-Way, LSD, p<0.05.
Results: The average number of osteoblast showed in Group 1 (9.7±3.6), Group 2 (20.7±0.5), Group 3 (32.46±0.557), Group 4 (40.73±0.894) and Group 5 (49.5±1.178). On the other hand, the average number of osteoclast showed in Group 1 (12.5±2.8), Group 2 (4.2±2.1), Group 3 (3.8±0.8), Group 4 (2.8±1.3) and Group 5 (2.0±0.9). In the comparison between the groups, there were significant differences for the number of osteoblast and osteoclast.
Conclusions:
It was revealed from our study that the combination graft material that contain with blood cockle’s shell and sea cucumber 1.6% has a good effect on the number of osteoblast and osteoclast in vivo.