IADR Abstract Archives
Chronic Apical Periodontitis Accelerates Inflammatory Response in Hyperlipidemic Rats
Objectives: This study aimed to investigate the effects of chronic apical periodontitis (CAP) on inflammatory immune responses and development of atherosclerosis in rats with hyperlipidemia.
Methods: Thirty-two male Sprague-Dawley (SD) rats were randomly divided into four groups (n=8). The control group (ND) was fed with regular diet. The ND+CAP group was treated with normal diet and CAP. The HFD group was supplied with high-fat diet. HFD+CAP group was given high-fat diet and CAP. The inflammatory responses of aorta were observed histologically using haematoxylin and sudan IV staining. The mRNA expressions of MCP-1, TLR2, TLR4, NF-κB p65 in aorta at 24th week were measured by quantitative Real-time PCR. Serum IL-2, IL-6, IL-10 and CRP were detected by Elisa.
Results: The ND and ND + CAP groups did not undergo evident aortic pathological damages, while foam cells appeared in the other two groups. Serum IL-6 and IL-2 levels were significantly elevated by individual high-fat diet or CAP stimulation (P < 0.05). Real-time PCR showed that TLR-2, TLR-4 and MCP-1 were highly expressed in the two HFD groups (P < 0.05). Western blotting showed that NF-κB p65 was expressed more highly in the three experimental groups than that in the ND group (P < 0.01).
Conclusions: CAP induced the high expressions of inflammatory factors and mediated inflammatory response. Although CAP elevated the blood lipid level less significantly than high-fat diet did, but it may promote atherosclerosis.
Methods: Thirty-two male Sprague-Dawley (SD) rats were randomly divided into four groups (n=8). The control group (ND) was fed with regular diet. The ND+CAP group was treated with normal diet and CAP. The HFD group was supplied with high-fat diet. HFD+CAP group was given high-fat diet and CAP. The inflammatory responses of aorta were observed histologically using haematoxylin and sudan IV staining. The mRNA expressions of MCP-1, TLR2, TLR4, NF-κB p65 in aorta at 24th week were measured by quantitative Real-time PCR. Serum IL-2, IL-6, IL-10 and CRP were detected by Elisa.
Results: The ND and ND + CAP groups did not undergo evident aortic pathological damages, while foam cells appeared in the other two groups. Serum IL-6 and IL-2 levels were significantly elevated by individual high-fat diet or CAP stimulation (P < 0.05). Real-time PCR showed that TLR-2, TLR-4 and MCP-1 were highly expressed in the two HFD groups (P < 0.05). Western blotting showed that NF-κB p65 was expressed more highly in the three experimental groups than that in the ND group (P < 0.01).
Conclusions: CAP induced the high expressions of inflammatory factors and mediated inflammatory response. Although CAP elevated the blood lipid level less significantly than high-fat diet did, but it may promote atherosclerosis.