IADR Abstract Archives
Collagenase and Simvastatin Containing Biphasic Biomaterial for Vital Pulp Therapy
Objectives: The aim was to develop a novel calcium phosphate/calcium sulfate (CPC-CSH) biphasic biomaterial with hyaluronic acid (HA) containing collagenase and simvastatin for vital pulp therapy (VPT).
Methods: Combination of HA with different ratios of CPC-CSH biphasic cements carrying collagenase and simvastatin was tested for setting time, degradation, and drug releasing ability. The hydration products and crystalline phases of the materials were characterized using scanning electron microscopy and energy-dispersive X-ray spectroscopy(SEM-EDS) analysis. In vitro biocompatibility was tested with human dental pulp stem cells (hDPSCs). Moreover, in vivo evaluation was done using a dog animal model by micro-CT evaluation and histological analysis.
Results: The results showed that the developed CPC-CSH cement, which contains 30 wt% CSH, exhibited apparent porosity, acceptable handling properties, and setting time for clinical use. The CPC-CSH cement showed a pronounced ability to form amorphous calcium phosphate (ACP) and dicalcium phosphate dehydrate (DCPD) crystals on the surface (SEM-EDS examinations). Results of alarmar blue and LDH revealed that hDPSCs treated with CPC-CSH cement containing collagenase and simvastatin was highly biocompatible. Animal study presented this CPC-CSH biphasic cement could induce calcified tissue with limited pulp irritation.
Conclusions: The results suggest that the CPC-CSH biphasic cement can be used for local delivery of collagenase and simvastatin and exhibits great potential for application in VPT.
Methods: Combination of HA with different ratios of CPC-CSH biphasic cements carrying collagenase and simvastatin was tested for setting time, degradation, and drug releasing ability. The hydration products and crystalline phases of the materials were characterized using scanning electron microscopy and energy-dispersive X-ray spectroscopy(SEM-EDS) analysis. In vitro biocompatibility was tested with human dental pulp stem cells (hDPSCs). Moreover, in vivo evaluation was done using a dog animal model by micro-CT evaluation and histological analysis.
Results: The results showed that the developed CPC-CSH cement, which contains 30 wt% CSH, exhibited apparent porosity, acceptable handling properties, and setting time for clinical use. The CPC-CSH cement showed a pronounced ability to form amorphous calcium phosphate (ACP) and dicalcium phosphate dehydrate (DCPD) crystals on the surface (SEM-EDS examinations). Results of alarmar blue and LDH revealed that hDPSCs treated with CPC-CSH cement containing collagenase and simvastatin was highly biocompatible. Animal study presented this CPC-CSH biphasic cement could induce calcified tissue with limited pulp irritation.
Conclusions: The results suggest that the CPC-CSH biphasic cement can be used for local delivery of collagenase and simvastatin and exhibits great potential for application in VPT.