IADR Abstract Archives
Microorganism Anti-Adhesive Efficiency of Sericin PEM Films Coated Titanium Discs
Objectives: Infection is a complication in dental implant surgery patients. Having biocompatibility, titanium can cause bacterial adhesion and biofilm formation. Biofilm is initial pathogenesis of infection in the area between implant and tissue. Currently, there are many studies on improving titanium properties on anti-microbial activity. Polyelectrolyte multilayer, or PEM, films are one of the technique used to improve titanium surface properties. It has been reported that sericin can promote osseointegration and anti-microbial activities. The aim of this study is to evaluate the inhibitory effect of sericin PEM films coated on titanium on adhesion of Staphylococcus aureus and Candida albicans.
Methods: The commercially pure titanium grade II (15mm diameter, 3mm thick) were prepared and coated with sericin at concentration of 0.1, 0.5, 1, 5, and 10%w/v by PEM technique. The adhesion of Candida albicans and Staphylococcus aureus was evaluated by adhesion assay, and cell adhesion morphology was examined by scanning electron microscope (SEM) analysis.
Results: The percentage of Candida albicans adhesion on titanium coated with sericin PEM films at concentration of 0.1 (46.74±11.94), 0.5 (56.09±12.75), 1 (51.61±17.92), 5 (47.92±17.95), and 10%w/v (47.64±9.59) were significantly less than those uncoated titanium surface (100.00±00.00). Although the percentage of Staphylococcus aureus adhesion on titanium coated with sericin PEM films at concentration of 0.1 (66.05±14.55), 0.5 (90.56±13.55), 1 (88.96±10.64), 5 (80.91±13.67), and 10%w/v (66.52±21.58) were less than the uncoated titanium (100.00±00.00), no statistically significant difference was observed. The result from SEM analysis was corresponded to the adhesion assay.
Conclusions: The titanium coated with sericin at concentration of 0.1, 0.5, 1, 5, and 10%w/v by PEM films technique are effective against of Candida albicans adhesion. Moreover, coating dental implant with sericin by PEM films technique can be alternative way to reduce infectious complication in dental implant surgery patients.
Methods: The commercially pure titanium grade II (15mm diameter, 3mm thick) were prepared and coated with sericin at concentration of 0.1, 0.5, 1, 5, and 10%w/v by PEM technique. The adhesion of Candida albicans and Staphylococcus aureus was evaluated by adhesion assay, and cell adhesion morphology was examined by scanning electron microscope (SEM) analysis.
Results: The percentage of Candida albicans adhesion on titanium coated with sericin PEM films at concentration of 0.1 (46.74±11.94), 0.5 (56.09±12.75), 1 (51.61±17.92), 5 (47.92±17.95), and 10%w/v (47.64±9.59) were significantly less than those uncoated titanium surface (100.00±00.00). Although the percentage of Staphylococcus aureus adhesion on titanium coated with sericin PEM films at concentration of 0.1 (66.05±14.55), 0.5 (90.56±13.55), 1 (88.96±10.64), 5 (80.91±13.67), and 10%w/v (66.52±21.58) were less than the uncoated titanium (100.00±00.00), no statistically significant difference was observed. The result from SEM analysis was corresponded to the adhesion assay.
Conclusions: The titanium coated with sericin at concentration of 0.1, 0.5, 1, 5, and 10%w/v by PEM films technique are effective against of Candida albicans adhesion. Moreover, coating dental implant with sericin by PEM films technique can be alternative way to reduce infectious complication in dental implant surgery patients.