IADR Abstract Archives
Conventional periodontal treatment and MPO secretion.
Objectives: : This study was undertaken to compare the activity and level of Myeloperoxidase (MPO) from saliva and from gingival crevicular fluid (GCF) in conventional treatment of periodontal disease.
Methods: : Saliva and GCF were collected from 24 subjects at before periodontal treatment, 2 weeks and 3 months after treatment :12 with chronic periodontitis (CP) and 12 with gingivitis. In addition, samples at baseline from 20 clinically healthy periodontium were collected. Full mouth plaque index (PI), bleeding on probing (BOP), periodontal pocket depth (PPD) and clinical attachment level (CAL) were also measured and recorded. MPO activity and level were measured by colorimetric method and indirect ELISA.
Results: MPO was constitutively secreted by neutrophil in oral cavity. Increase in MPO activity, and level was seen in patients with chronic periodontitis. Significant difference in MPO was seen in both saliva and GCF of periodontally healthy and gingivitis patients compared with patients with periodontitis. After scaling and root planing, MPO concentration and activity was significant changed in both gingivitis and periodontitis groups.
Conclusions: MPO activity, MPO level and ratio between MPO to total protein from GCF or saliva might be considered as a prognostic biomarker of periodontal inflammation. Conventional treatment of periodontal disease altered the MPO activity, level and ratio of MPO to total protein in inflamed periodontium.
Methods: : Saliva and GCF were collected from 24 subjects at before periodontal treatment, 2 weeks and 3 months after treatment :12 with chronic periodontitis (CP) and 12 with gingivitis. In addition, samples at baseline from 20 clinically healthy periodontium were collected. Full mouth plaque index (PI), bleeding on probing (BOP), periodontal pocket depth (PPD) and clinical attachment level (CAL) were also measured and recorded. MPO activity and level were measured by colorimetric method and indirect ELISA.
Results: MPO was constitutively secreted by neutrophil in oral cavity. Increase in MPO activity, and level was seen in patients with chronic periodontitis. Significant difference in MPO was seen in both saliva and GCF of periodontally healthy and gingivitis patients compared with patients with periodontitis. After scaling and root planing, MPO concentration and activity was significant changed in both gingivitis and periodontitis groups.
Conclusions: MPO activity, MPO level and ratio between MPO to total protein from GCF or saliva might be considered as a prognostic biomarker of periodontal inflammation. Conventional treatment of periodontal disease altered the MPO activity, level and ratio of MPO to total protein in inflamed periodontium.