IADR Abstract Archives
Remineralization potential of three toothpastes on initial carious lesions: An in-vitro pH-cycling study
Objectives: This in-vitro study evaluated the remineralization potential of 3 different toothpastes on initial enamel lesion using surface microhardness (SMH).
Methods: A total of 40 extracted sound human upper premolars were decoronated, coated with nail varnish except for a 4mmx4mm window on buccal surface. Artificial enamel carious lesions were created by immersing specimens in demineralizing solution for 96h. SMH of demineralized specimens were measured using Vickers microhardness testing machine. Specimens were randomly assigned into 4 groups (n=10); Group 1: artificial saliva (control), Group 2: sodium fluoride (NaF) containing toothpaste, Group 3: NaF and functionalized tricalcium phosphate (NaF/f-TCP) containing toothpaste and Group 4; NaF and calcium sodium phosphosilicate (NaF/CSP) containing toothpaste. 10 days of pH cycling regimen was carried out, SMH of remineralized specimens were evaluated and calculated for difference of SMH (dSMH). The mean-dSMH of all groups were statistically analyzed (One-way ANOVA, Bonferroni, p<0.05).
Results: Mean-dSMH of group 1, 2, 3 and 4 were -2.47±0.17, 37.28±11.04, 44.84±12.18 and 37.14±10.12 kgf respectively. The results showed that mean-dSMH of group 2, 3 and 4 were significantly higher than that of group1 (p<0.02). No statistically significant difference in mean-dSMH was found among group 2, 3 and 4 (p=0.356).
Conclusions: With the in-vitro conditions chosen, toothpastes containing NaF, NaF/f-TCP and NaF/CSP performed the remineralization potential of initial enamel carious lesions, while no significant difference was observed between three toothpastes.
Methods: A total of 40 extracted sound human upper premolars were decoronated, coated with nail varnish except for a 4mmx4mm window on buccal surface. Artificial enamel carious lesions were created by immersing specimens in demineralizing solution for 96h. SMH of demineralized specimens were measured using Vickers microhardness testing machine. Specimens were randomly assigned into 4 groups (n=10); Group 1: artificial saliva (control), Group 2: sodium fluoride (NaF) containing toothpaste, Group 3: NaF and functionalized tricalcium phosphate (NaF/f-TCP) containing toothpaste and Group 4; NaF and calcium sodium phosphosilicate (NaF/CSP) containing toothpaste. 10 days of pH cycling regimen was carried out, SMH of remineralized specimens were evaluated and calculated for difference of SMH (dSMH). The mean-dSMH of all groups were statistically analyzed (One-way ANOVA, Bonferroni, p<0.05).
Results: Mean-dSMH of group 1, 2, 3 and 4 were -2.47±0.17, 37.28±11.04, 44.84±12.18 and 37.14±10.12 kgf respectively. The results showed that mean-dSMH of group 2, 3 and 4 were significantly higher than that of group1 (p<0.02). No statistically significant difference in mean-dSMH was found among group 2, 3 and 4 (p=0.356).
Conclusions: With the in-vitro conditions chosen, toothpastes containing NaF, NaF/f-TCP and NaF/CSP performed the remineralization potential of initial enamel carious lesions, while no significant difference was observed between three toothpastes.