IADR Abstract Archives
The bone biological effects of local anesthesia drugs
Objectives: The purpose of this study was to investigate the drug of local anesthesia effects on the osteogenic effects
Methods: The Scandonest 3% (mepivacaine hydrochloride, Septodont Co. , S-) , Septanest with adrenaline (Articaine hydrochloride , Septodont Co, S+)of the local anesthesia were used in present study. The human periodontal ligament cell line (PDL) were as target to test the cellular changes. The various concentration of the anesthesia drugs (0. 2.5 ,5,10 um) were added to the PDL culture plates. After 24 hrs cultured, the cultured PDL extracts were added to the osteroblast cell line (MG63). After cultured 24 hrs, the osteoblast differentiation markers RANKL and ALP expression were analysed by western blot analysis.
Results: The PDL or MG63 cell morphologies were no changes as compared with control groups. As the concentration of extracts increased, the RANKL expression were decreased on S- and S+ group , but no effect on ALP expression.The The RANKL and ALP expression were decreased under the 10 um of S- group. (p<0.05),
Conclusions: From the present study, the local anesthesia drugs without adrenaline can affect the bone cell differentiation ability on the RANKL expression.
Methods: The Scandonest 3% (mepivacaine hydrochloride, Septodont Co. , S-) , Septanest with adrenaline (Articaine hydrochloride , Septodont Co, S+)of the local anesthesia were used in present study. The human periodontal ligament cell line (PDL) were as target to test the cellular changes. The various concentration of the anesthesia drugs (0. 2.5 ,5,10 um) were added to the PDL culture plates. After 24 hrs cultured, the cultured PDL extracts were added to the osteroblast cell line (MG63). After cultured 24 hrs, the osteoblast differentiation markers RANKL and ALP expression were analysed by western blot analysis.
Results: The PDL or MG63 cell morphologies were no changes as compared with control groups. As the concentration of extracts increased, the RANKL expression were decreased on S- and S+ group , but no effect on ALP expression.The The RANKL and ALP expression were decreased under the 10 um of S- group. (p<0.05),
Conclusions: From the present study, the local anesthesia drugs without adrenaline can affect the bone cell differentiation ability on the RANKL expression.