IADR Abstract Archives
Effects of Erythrosine+Nano-Tianium Dioxide-Mediated Photodynamic Therapy on HGF-1 Cells
Objectives: To study the effects of erythrosine+Nano-TiO2 as a photosensitizer on viability and morphology of normal human gingival fibroblast (HGF-1) cells.
Methods:
Eleven test groups were studied, namely, Erythrosine (Ery)at 0, 55 µM, 110 µM, 220µM, 440µM in the presence and or absence of 1% nano TiO2 + blue light, Nano-TiO2 alone, and control groups (+ve control = DMEM, -ve control = H2O2). Normal human gingival fibroblast (ATCC CRL-2014, HGF-1) were cultured in 96-well plate standard condition (37°C, 5% CO2, 95 percent humidity, 24hrs) Subsequently, a photosensitizer was applied to experimental groups for 15 min. Test groups were irradiated with LED-dental curing-light (BA Optima 10, 420-480 nm, 15 J/cm2) for 1 min,at 1, 6 or 24 hrs, cell viability assays then were performed using PrestoBlue (Invitrogen, Life Technologies GmbH, Darmstadt Germany) and analysis of absorbance were accomplished byVarioskan™ at excitation/emission wavelength 560/620 nm. Cell morphology of all groups was investigated by SEM (S3000N, Hitashi, Japan).
Results:
HGF-1 cell viability were not statistical significance different among all groups at all time point by ANOVA and post hoc test. Minor morphology change were observed between test and control groups by SEM.
Conclusions:
Erythrosine with nano-titanium dioxide, a novel PDT photosensitizer, in the range of the present study exerted no toxicity on HGF-1 cell.
Methods:
Eleven test groups were studied, namely, Erythrosine (Ery)at 0, 55 µM, 110 µM, 220µM, 440µM in the presence and or absence of 1% nano TiO2 + blue light, Nano-TiO2 alone, and control groups (+ve control = DMEM, -ve control = H2O2). Normal human gingival fibroblast (ATCC CRL-2014, HGF-1) were cultured in 96-well plate standard condition (37°C, 5% CO2, 95 percent humidity, 24hrs) Subsequently, a photosensitizer was applied to experimental groups for 15 min. Test groups were irradiated with LED-dental curing-light (BA Optima 10, 420-480 nm, 15 J/cm2) for 1 min,at 1, 6 or 24 hrs, cell viability assays then were performed using PrestoBlue (Invitrogen, Life Technologies GmbH, Darmstadt Germany) and analysis of absorbance were accomplished byVarioskan™ at excitation/emission wavelength 560/620 nm. Cell morphology of all groups was investigated by SEM (S3000N, Hitashi, Japan).
Results:
HGF-1 cell viability were not statistical significance different among all groups at all time point by ANOVA and post hoc test. Minor morphology change were observed between test and control groups by SEM.
Conclusions:
Erythrosine with nano-titanium dioxide, a novel PDT photosensitizer, in the range of the present study exerted no toxicity on HGF-1 cell.