IADR Abstract Archives
Effects of 810nm Diode-Laser on Human Gingival Fibroblasts Proliferation and Migration.
Objectives: The objective of this study was to evaluate the effects of 810nm diode-laser on proliferation and migration of the human gingival fibroblasts (hGF) according to different settings.
Methods: hGF was obtained from gingival tissue in the maxillary anterior tooth region of healthy donors. The cell monolayer was irradiated with 810nm diode laser which operated at different parameters following manufacturer’s instructions: group 1- elimination of inflamed tissue and pocket epithelium; group 2- disinfection; group 3- biostimulation and group 4- control group (non-irradiated). Cell proliferation was evaluated by MTT assay after 1, 3, 5, 7, 9 days. Cell migration was evaluated by scratch wound healing assay at 0, 24, 48, 72 h. Kruskal-Wallis test and Mann-Whitney one were applied to analyze these data.
Results: Cells in group 1 had lowest proliferation at all-time points. Cells in group 3 showed a better proliferation than those in the remaining groups at day 1, day 5 and day 7 and a lower proliferation than the control group at the day 3 and day 9. However, the difference in cell proliferation among groups was only statistically significant at day 9 (p<0.001). At this time point, cells in both group 2 and group 3 showed a significant increase in comparison with group 1 (p<0.05) but no difference compared to control one (p>0.05). The migration rate of cells in group 3 was faster than the other groups (48 hours versus 72 hours after irradiation).
Conclusions: Laser can modulate proliferation and migration of hGF. This study contributed more evidence for possible effects of laser on wound healing process after periodontal treatment.
Methods: hGF was obtained from gingival tissue in the maxillary anterior tooth region of healthy donors. The cell monolayer was irradiated with 810nm diode laser which operated at different parameters following manufacturer’s instructions: group 1- elimination of inflamed tissue and pocket epithelium; group 2- disinfection; group 3- biostimulation and group 4- control group (non-irradiated). Cell proliferation was evaluated by MTT assay after 1, 3, 5, 7, 9 days. Cell migration was evaluated by scratch wound healing assay at 0, 24, 48, 72 h. Kruskal-Wallis test and Mann-Whitney one were applied to analyze these data.
Results: Cells in group 1 had lowest proliferation at all-time points. Cells in group 3 showed a better proliferation than those in the remaining groups at day 1, day 5 and day 7 and a lower proliferation than the control group at the day 3 and day 9. However, the difference in cell proliferation among groups was only statistically significant at day 9 (p<0.001). At this time point, cells in both group 2 and group 3 showed a significant increase in comparison with group 1 (p<0.05) but no difference compared to control one (p>0.05). The migration rate of cells in group 3 was faster than the other groups (48 hours versus 72 hours after irradiation).
Conclusions: Laser can modulate proliferation and migration of hGF. This study contributed more evidence for possible effects of laser on wound healing process after periodontal treatment.