IADR Abstract Archives
Dental Pulp Cell Viability After Exposed to Hydroxyapatite Synthesized from Yellowfin Tuna Bone
Objectives: Yellowfin tuna (Thunnus albacares) is one of the most widely consumed fish in Indonesia. The bone of the fish contains high calcium and phosphor and can be synthesized into hydroxyapatite (HA). Due to its mineral content and similarity in chemical composition with human hard tissue, HA may have a potency as a pulp capping material. The aim of this study was to evaluate the viability of dental pulp cells after exposed to HA synthesized from yellowfin tuna bone.
Methods: Pulp cells were isolated from human third molar. The cells were cultured in DMEM supplemented with 10% FBS, 2% Penicillin and Streptomycin, and 0.5% fungizone. The cells were exposed to various concentrations (200 to 3.125 μg/ml) of HA for 24, 48, 72, 96, and 120 hours. The viability of the cells was examined using MTT assay and the morphology of the cells was observed under a fluorescence microscope after stained with ethidium bromide acridine orange. All of the experiments were conducted in triplicate.
Results: Results showed that the viability of the cells was 92%-97% after exposed to HA for 24 hours. Cell viability was increased slightly over the time. After 120 hours, the viability of the cells increased to 101%-118%. Morphological observation showed neither nuclear changes nor apoptotic body formation.
Conclusions: In conclusion, dental pulp cells maintain their viability after exposed to HA synthesized from yellowfin tuna bone.
Methods: Pulp cells were isolated from human third molar. The cells were cultured in DMEM supplemented with 10% FBS, 2% Penicillin and Streptomycin, and 0.5% fungizone. The cells were exposed to various concentrations (200 to 3.125 μg/ml) of HA for 24, 48, 72, 96, and 120 hours. The viability of the cells was examined using MTT assay and the morphology of the cells was observed under a fluorescence microscope after stained with ethidium bromide acridine orange. All of the experiments were conducted in triplicate.
Results: Results showed that the viability of the cells was 92%-97% after exposed to HA for 24 hours. Cell viability was increased slightly over the time. After 120 hours, the viability of the cells increased to 101%-118%. Morphological observation showed neither nuclear changes nor apoptotic body formation.
Conclusions: In conclusion, dental pulp cells maintain their viability after exposed to HA synthesized from yellowfin tuna bone.