IADR Abstract Archives
Biocompatibility of resin-based sealant prototype, LAS-opaque and-clear, in subcutaneous tissue
Objectives: To evaluate the biocompatibility of the light-activated pit and fissure resin-based dental sealant prototypes, LAS-clear and LAS-opaque, after subcutaneous implantation in rats for 7 and 60 days.
Methods: The commercial light activated resin-based sealants, Delton-clear (DC), Helioseal-clear (HC), Helioseal-opaque (HO), and Clinpro-Sealant (CL) served as control. Fourteen 8-week old Sprague-Dawley rats were used. Two rows of three 5 mm subcutaneous incisions were created on the back of each rat parallel to the midline. The materials were randomly implanted and the incisions were closed using sutures. The implants were left in situ for 7 and 60 days. The implanted samples and the surrounding skin were removed, sectioned, and stained for histopathological evaluation: inflammatory cell infiltration (ICI) and tissue response (TR). The histopathological scores were analyzed by the Kruskal-Wallis and Bonferroni multiple comparison tests (p<0.05).
Results: The average ICI score between DC, HC, LAS-clear, HO, CL, and LAS-opaque were not significant difference at 7 and 60 days post-operation (p>0.05). The average ICI score of DC, HC, LAS-clear, HO, CL, and LAS-opaque for 7 and 60 days post-operation were 3.5±1.41, 3.63±0.74, 3.13±0.83, 3.25±1.04, 3.13±0.99, and 3.13±1.25 and were 2.33±1.15, 2.33±1.15, 2.83±1.03, 2.38±0.76, 2.23±0.83, and 2.41±0.79, respectively. Although all the material revealed a persistent inflammatory response mediated by lymphocytes and macrophages at all time point evaluation, the tissue reaction of all groups to material implantation was that of typical wound healing. The tissue response score between DC, HC, LAS-clear, HO, CL, and LAS-opaque were also not significant difference at 7 and 60 days post-implantation. The average TR score ofDC, HC, LAS-clear, HO, CL, and LAS-opaque for 7 and 60 days post-operation were 1.38±0.52, 1.38±0.51, 1.38±0.52, 1.25±0.46, 1.38±0.52, and 1.25±0.46 and were 2.0, 1.91±0.29, 2.0, 1.62±0.51, 1.62±0.51, and 1.83±0.39, respectively. The tissue response we observed was material encapsulation without marked pathology. The capsule thickness was gradually increased in time depending manner.
Conclusions: LAS-clear and LAS-opaque materials were biocompatible with the subcutaneous layer.
Methods: The commercial light activated resin-based sealants, Delton-clear (DC), Helioseal-clear (HC), Helioseal-opaque (HO), and Clinpro-Sealant (CL) served as control. Fourteen 8-week old Sprague-Dawley rats were used. Two rows of three 5 mm subcutaneous incisions were created on the back of each rat parallel to the midline. The materials were randomly implanted and the incisions were closed using sutures. The implants were left in situ for 7 and 60 days. The implanted samples and the surrounding skin were removed, sectioned, and stained for histopathological evaluation: inflammatory cell infiltration (ICI) and tissue response (TR). The histopathological scores were analyzed by the Kruskal-Wallis and Bonferroni multiple comparison tests (p<0.05).
Results: The average ICI score between DC, HC, LAS-clear, HO, CL, and LAS-opaque were not significant difference at 7 and 60 days post-operation (p>0.05). The average ICI score of DC, HC, LAS-clear, HO, CL, and LAS-opaque for 7 and 60 days post-operation were 3.5±1.41, 3.63±0.74, 3.13±0.83, 3.25±1.04, 3.13±0.99, and 3.13±1.25 and were 2.33±1.15, 2.33±1.15, 2.83±1.03, 2.38±0.76, 2.23±0.83, and 2.41±0.79, respectively. Although all the material revealed a persistent inflammatory response mediated by lymphocytes and macrophages at all time point evaluation, the tissue reaction of all groups to material implantation was that of typical wound healing. The tissue response score between DC, HC, LAS-clear, HO, CL, and LAS-opaque were also not significant difference at 7 and 60 days post-implantation. The average TR score ofDC, HC, LAS-clear, HO, CL, and LAS-opaque for 7 and 60 days post-operation were 1.38±0.52, 1.38±0.51, 1.38±0.52, 1.25±0.46, 1.38±0.52, and 1.25±0.46 and were 2.0, 1.91±0.29, 2.0, 1.62±0.51, 1.62±0.51, and 1.83±0.39, respectively. The tissue response we observed was material encapsulation without marked pathology. The capsule thickness was gradually increased in time depending manner.
Conclusions: LAS-clear and LAS-opaque materials were biocompatible with the subcutaneous layer.