IADR Abstract Archives
Antioxidant Activity of Various Oral Lactobacillus Strains
Objectives: Objectives: This study aims to evaluate the antioxidant activity of various Lactobacillus strains derived from human oral cavity an in vitro.
Methods: A total of 201 Lactobacillus strains were investigated for antioxidant activities: free radical scavenging (DPPH), inhibition of linoleic acid peroxidation (TAALA), superoxide dismutase (SOD) and glutathione (GSH). Resistance to reactive oxygen species was also assessed. A correlation of antioxidant activities and dental status were examined. Differences for median values of DPPH, TAALA, SOD and GSH among the Lactobacillus species were analyzed using the Kruskal-Wallis test followed by the Mann-Whitney U test.
Results: It showed that there was a wide range of individual antioxidant properties of DPPH, TAALA, SOD and GSH between/within the species of Lactobacillus strains. Generally, all tested Lactobacillus, except L. oris and L. gasseri, showed relatively high antioxidative parameters indicating that antioxidative properties are strain specific. L. fermentum, L. paracasei and L. rhamnosus strains with high DPPH and TAALA activities (>60%) could survive under oxidative stress (incubated in 1.0 mmol/l hydrogen peroxide for 8 h or 1.0 mmol/l hydroxyl radicals for 1 h) better than L. salivarius, L. oris and L. gasseri strains. It was also observed that more than 50% of caries free strains (25 isolates from 9 caries free subjects) possessed higher antioxidant than caries strains (176 isolates from 79 caries subjects).
Conclusions: This is the first study to investigate the antioxidant properties of human oral Lactobacillus strains. The different antioxidants were found among Lactobacillus strains. The strains with high antioxidants could tolerate well under the oxidative stress. Significant results were found that the certain Lactobacillus strains might be a source of antioxidants. Further studies in animals and humans are needed to prove their benefits in promoting oral health.
Methods: A total of 201 Lactobacillus strains were investigated for antioxidant activities: free radical scavenging (DPPH), inhibition of linoleic acid peroxidation (TAALA), superoxide dismutase (SOD) and glutathione (GSH). Resistance to reactive oxygen species was also assessed. A correlation of antioxidant activities and dental status were examined. Differences for median values of DPPH, TAALA, SOD and GSH among the Lactobacillus species were analyzed using the Kruskal-Wallis test followed by the Mann-Whitney U test.
Results: It showed that there was a wide range of individual antioxidant properties of DPPH, TAALA, SOD and GSH between/within the species of Lactobacillus strains. Generally, all tested Lactobacillus, except L. oris and L. gasseri, showed relatively high antioxidative parameters indicating that antioxidative properties are strain specific. L. fermentum, L. paracasei and L. rhamnosus strains with high DPPH and TAALA activities (>60%) could survive under oxidative stress (incubated in 1.0 mmol/l hydrogen peroxide for 8 h or 1.0 mmol/l hydroxyl radicals for 1 h) better than L. salivarius, L. oris and L. gasseri strains. It was also observed that more than 50% of caries free strains (25 isolates from 9 caries free subjects) possessed higher antioxidant than caries strains (176 isolates from 79 caries subjects).
Conclusions: This is the first study to investigate the antioxidant properties of human oral Lactobacillus strains. The different antioxidants were found among Lactobacillus strains. The strains with high antioxidants could tolerate well under the oxidative stress. Significant results were found that the certain Lactobacillus strains might be a source of antioxidants. Further studies in animals and humans are needed to prove their benefits in promoting oral health.