IADR Abstract Archives
Effect of Iloprost in Wound Healing in Dental Pulp under Hypoxia
Objectives: Iloprost is the analogue of prostacyclin (PGI2) and was used in clinical treatment related to vasculogenesis. The effect of iloprost in human dental pulp cells (HDPCs) to hypoxic conditions is not knowm. This study aimed to investigate the effect of iloprost on angiogenic, osteo/odontogenic and inflammatory gene expression in HDPCs in hypoxic condition in vitro.
Methods: HDPCs were subjected to normoxic and hypoxic (2% O2) condition for 1 day. Hypoxic conditions was created in an airtight chamber de-oxygenated with the constant infusion with humidified gas mixtures (n=3). The RNA samples were extracted and cDNA was synthesized. The qPCR analysis was performed. The scratch test assay was performed on HDPCs at 0, 24 and 72 h to determine wound healing effect of iloprost. The ANOVA statistics was performed at p<0.05.
Results: From this study, iloprost up-regulated VEGF expression in normoxia, but did not further up-regulated VEGF in hypoxic condition of HDPCs at 24 hours. The increase of VEGF was down-regulated by the treatment of iloprost. Hypoxia up-regulated BSP and was further up-regulated by iloprost. DMP-1 was down-regulated in hypoxia, while iloprost treatment up-regulated DMP-1 in hypoxia. Hypoxia up-regulated IL-6, but iloprost treatment decreased the effect of hypoxia on IL-6 expression. Iloprost enhanced the wound healing effect in scratch essay at 72 h by significant decrease of the wound closure.
Conclusions: Angiogenic and osteo/odontogenic signaling during hypoxic condition play a key role in the formation of reparative dentin. Iloprost improved the dental pulp healing in hypoxic condition by increasing osteo/odontogenic markers and reduced inflammatory gene markers. Iloprost also increased the healing rate of wound healing in scratch essay. The treatment of iloprost might be another therapeutic alternative for patients with dental trauma.
Methods: HDPCs were subjected to normoxic and hypoxic (2% O2) condition for 1 day. Hypoxic conditions was created in an airtight chamber de-oxygenated with the constant infusion with humidified gas mixtures (n=3). The RNA samples were extracted and cDNA was synthesized. The qPCR analysis was performed. The scratch test assay was performed on HDPCs at 0, 24 and 72 h to determine wound healing effect of iloprost. The ANOVA statistics was performed at p<0.05.
Results: From this study, iloprost up-regulated VEGF expression in normoxia, but did not further up-regulated VEGF in hypoxic condition of HDPCs at 24 hours. The increase of VEGF was down-regulated by the treatment of iloprost. Hypoxia up-regulated BSP and was further up-regulated by iloprost. DMP-1 was down-regulated in hypoxia, while iloprost treatment up-regulated DMP-1 in hypoxia. Hypoxia up-regulated IL-6, but iloprost treatment decreased the effect of hypoxia on IL-6 expression. Iloprost enhanced the wound healing effect in scratch essay at 72 h by significant decrease of the wound closure.
Conclusions: Angiogenic and osteo/odontogenic signaling during hypoxic condition play a key role in the formation of reparative dentin. Iloprost improved the dental pulp healing in hypoxic condition by increasing osteo/odontogenic markers and reduced inflammatory gene markers. Iloprost also increased the healing rate of wound healing in scratch essay. The treatment of iloprost might be another therapeutic alternative for patients with dental trauma.