IADR Abstract Archives
The Effect of Mouthrinse Containing Immunolobulin-Y Anti ComD S.mutans towards S.mutans Biofilm Formation
Objectives: The bond between protein surface of S.mutans with IgY can reduce pathogenic properties of S.mutans. The effectiveness of IgY as a dental caries vaccine should be improved by creating IgY specific to ComD that has a role in the mechanism of quorum sensing. To determine the effects of mouth rinse containing IgY Anti ComD S.mutans towards the ability of S.mutans isolated from caries and free caries subjects to form biofilm.
Methods: DMFT scores of subjects were measured and divided into 2 groups: caries group and free caries group. Subjects were assigned to rinse twice daily (for 6 days) with 15-20 ml solution of mouth rinse for 30 seconds containing IgY Anti-ComD S.mutans. Plaque samples were taken from the buccal surface of the tooth in each subjects before and after using mouth rinse. S.mutans samples were taken from the plaque culture medium of each subjects. Then crystal violet biofilm test procedure was performed using microplate reader to obtain the value of optical density (OD).
Results: The average of biofilm mass (OD values) before application of mouth rinse containing IgY Anti ComD S.mutans in free caries subjects is 0,163 and in caries subjects 0,153. There is a reduction of biofilm formation after administration of mouth rinse containing IgY Anti ComD S.mutansin free caries subjects 0,141 and in caries subjects 0,136 (p> 0.05).
Conclusions: Mouth rinse containing IgY Anti-ComD S.mutans could decrease S.mutans biofilm formation isolated from caries and free caries subjects.
Methods: DMFT scores of subjects were measured and divided into 2 groups: caries group and free caries group. Subjects were assigned to rinse twice daily (for 6 days) with 15-20 ml solution of mouth rinse for 30 seconds containing IgY Anti-ComD S.mutans. Plaque samples were taken from the buccal surface of the tooth in each subjects before and after using mouth rinse. S.mutans samples were taken from the plaque culture medium of each subjects. Then crystal violet biofilm test procedure was performed using microplate reader to obtain the value of optical density (OD).
Results: The average of biofilm mass (OD values) before application of mouth rinse containing IgY Anti ComD S.mutans in free caries subjects is 0,163 and in caries subjects 0,153. There is a reduction of biofilm formation after administration of mouth rinse containing IgY Anti ComD S.mutansin free caries subjects 0,141 and in caries subjects 0,136 (p> 0.05).
Conclusions: Mouth rinse containing IgY Anti-ComD S.mutans could decrease S.mutans biofilm formation isolated from caries and free caries subjects.