IADR Abstract Archives
Aragan (Turbinaria conoides) as a Potential Bone Grafting Material
Objectives: This study aims to determine the potential of Aragan as a bone grafting material.
Methods: Right upper and lower 1st molars of 16 male Sprague Dawley rats were extracted. Using randomization, experimental and control sites were assigned. Aragan, prepared by lyophilization, pulverization and sterilization was tightly packed on experimental site. Test animals were cultured in the same environment and feeding procedures. Three rats were subjected to euthanasia and histological analysis was done after 7, 14, 21 and 28 days. Mean and Standard Deviation, Frequency and Percentage, Independent t-test, Fisher’s exact test were utilized to interpret the results.
Results: Inflammatory response was present only on the first week of both test sites. Fibroblast proliferation was relatively higher in control sites than the experimental sites though statistical tests reveal no significant difference because of the small samples. Vascularization developed among experimental sites after seven days by 13% (2.83 vs 2.50) than the control, after 14 days by 19% (2.17 vs 1.83). Vascularization, after 21 days is relatively higher in control sites than the experimental sites by 7% (2.33 vs 2.17), after 28 days by 14% (2.33 vs 2). While statistical test reveals no significant difference with the control, the experimental group have consistently 100% presence of chondrocytes from first to fourth week. Presence of osteoblast is relatively higher among control group but not significant (p=0.200>0.05) after 7 and 14 days, respectively. However, experimental group had 100% presence of osteoblast from 14 to 28 days. This is 33% higher than control group but not significant. Though, no significant difference, there is a relatively higher percentage of bone formation present among experimental than control group.
Conclusions: Aragan is a biocompatible and can be used as an alternative grafting material.
Methods: Right upper and lower 1st molars of 16 male Sprague Dawley rats were extracted. Using randomization, experimental and control sites were assigned. Aragan, prepared by lyophilization, pulverization and sterilization was tightly packed on experimental site. Test animals were cultured in the same environment and feeding procedures. Three rats were subjected to euthanasia and histological analysis was done after 7, 14, 21 and 28 days. Mean and Standard Deviation, Frequency and Percentage, Independent t-test, Fisher’s exact test were utilized to interpret the results.
Results: Inflammatory response was present only on the first week of both test sites. Fibroblast proliferation was relatively higher in control sites than the experimental sites though statistical tests reveal no significant difference because of the small samples. Vascularization developed among experimental sites after seven days by 13% (2.83 vs 2.50) than the control, after 14 days by 19% (2.17 vs 1.83). Vascularization, after 21 days is relatively higher in control sites than the experimental sites by 7% (2.33 vs 2.17), after 28 days by 14% (2.33 vs 2). While statistical test reveals no significant difference with the control, the experimental group have consistently 100% presence of chondrocytes from first to fourth week. Presence of osteoblast is relatively higher among control group but not significant (p=0.200>0.05) after 7 and 14 days, respectively. However, experimental group had 100% presence of osteoblast from 14 to 28 days. This is 33% higher than control group but not significant. Though, no significant difference, there is a relatively higher percentage of bone formation present among experimental than control group.
Conclusions: Aragan is a biocompatible and can be used as an alternative grafting material.