IADR Abstract Archives
Effect of mouthrinses on the adhering capacity of individual and mixed bacterial biofilm
Objectives: To compare the effect of commercial mouthrinses on the adhering capacity of selected oral bacteria (Strep mitis,Strep sanguinis,Actinomyces viscosus) to the experimental pellicle.
Methods: NAM model was used to develop the oral biofilm in dynamic state. Saliva was pumped through the glass tube containing glass beads of the NAM model at 0.3 ml/sec followed by deionised distilled water (remove excess saliva) to form the experimental pellicle. Mouthrinse (2x dilution except for 0.046% Eucalyptol and 0.032% Thymol) was pumped over the glass beads in the NAM model (to treat the experimental pellicle) followed by deionised distilled water through the tubing to rinse any excess mouthrinse. The treated experimental pellicle was subsequently inoculated with the bacterial suspension (Strep. mitis( S.m), Strep.sanguinis (S.s) and Actinomyces viscosus (A.v) ) respectively and allowed it to flow into the system for 24 hrs at 37oC. Glass beads (n=3) were collected from the NAM model. The adhered cells on each bead were determined and expressed as colony forming units per ml (CFU/mL). The experiment was repeated using mixture of bacteria (A.v+S.s, A.v+S.m). In this study three types of mouth rinses were used to treat the experimental pellicle their active ingredients were cetylpyridinium chloride (0.0375%), essential oil(0.046% Eucalyptol and 0.032% Thymol) and chlorhexidine gluconate(0.06%). Deionized distilled water was used as the negative control. Each bead was analysed for CFU in triplicates and the experiment was repeated three times (final n=27), results were analyzed using SPSS statistical software version 19. Non parametric (Kruskal wallis) test was used.
Results: The results demonstrated that active ingredient 0.0375% cetylpyridinium chloride (CPC) containing mouthrinse was more effective than 0.06% chlorhexidine gluconate (CHX), 0.046% Eucalyptol, and 0.032% Thymol (Kruskal Wallis test, p<0.05).
Conclusions: In conclusion, 0.0375% Cetylpyridinium chloride (CPC) and 0.06% chlorhexidine gluconate (CHX) significantly reduces the percentage of adhered bacterial cells even at 2x dilution compared to Essential Oil (E.O) *(without dilution).
Methods: NAM model was used to develop the oral biofilm in dynamic state. Saliva was pumped through the glass tube containing glass beads of the NAM model at 0.3 ml/sec followed by deionised distilled water (remove excess saliva) to form the experimental pellicle. Mouthrinse (2x dilution except for 0.046% Eucalyptol and 0.032% Thymol) was pumped over the glass beads in the NAM model (to treat the experimental pellicle) followed by deionised distilled water through the tubing to rinse any excess mouthrinse. The treated experimental pellicle was subsequently inoculated with the bacterial suspension (Strep. mitis( S.m), Strep.sanguinis (S.s) and Actinomyces viscosus (A.v) ) respectively and allowed it to flow into the system for 24 hrs at 37oC. Glass beads (n=3) were collected from the NAM model. The adhered cells on each bead were determined and expressed as colony forming units per ml (CFU/mL). The experiment was repeated using mixture of bacteria (A.v+S.s, A.v+S.m). In this study three types of mouth rinses were used to treat the experimental pellicle their active ingredients were cetylpyridinium chloride (0.0375%), essential oil(0.046% Eucalyptol and 0.032% Thymol) and chlorhexidine gluconate(0.06%). Deionized distilled water was used as the negative control. Each bead was analysed for CFU in triplicates and the experiment was repeated three times (final n=27), results were analyzed using SPSS statistical software version 19. Non parametric (Kruskal wallis) test was used.
Results: The results demonstrated that active ingredient 0.0375% cetylpyridinium chloride (CPC) containing mouthrinse was more effective than 0.06% chlorhexidine gluconate (CHX), 0.046% Eucalyptol, and 0.032% Thymol (Kruskal Wallis test, p<0.05).
Conclusions: In conclusion, 0.0375% Cetylpyridinium chloride (CPC) and 0.06% chlorhexidine gluconate (CHX) significantly reduces the percentage of adhered bacterial cells even at 2x dilution compared to Essential Oil (E.O) *(without dilution).