IADR Abstract Archives
In-vitro cariogenicity of isomaltulose and sucrose under multi-species biofilm
Objectives: To compare the in-vitro cariogenicity of isomaltulose and sucrose on human tooth infected with multi-species biofilm.
Methods: Sixty-four tooth blocks with size of 2.0x2.0x4.0 mm were prepared with 1.0x1.0 mm windows on enamel and dentine by applying acid resistant nail varnish on other surfaces. The blocks were randomly allocated into four groups, 16 each, and placed in media containing (1) 10% isomaltulose, (2) 20% isomaltulose, (3) 10% sucrose, or (4) 20% sucrose, in brain-heart Infusion (BHI) broth. Four cariogenic oral bacteria, Streptococcus mutans, Streptococcus sobrinus, Lactobacillus acidophilus and Lactobacillus rhamnosus, were added to the media to allow biofilm formation and creation of artificial caries lesions in the tooth blocks. These were then incubated anaerobically at 37°C. After every 24 hours, the medium was refreshed manually. Depth of artificial caries lesions created after seven days was measured by micro-CT scanning.
Results: Artificial caries lesions in enamel were found in most of the tooth blocks in the sucrose groups (12 in 10% group and 10 in 20% group), but in only 2 blocks in each the isomaltulose groups (p<0.001). Nearly all of the tooth blocks developed caries lesion in dentine. The mean depth of the lesions in dentine in the 10% isomaltulose group was smaller than that of the 10% sucrose group (149.0±36.0 µm vs. 216.6±26.7 µm, p<0.001). Shallower lesions in the 20% isomaltulose group than in the 20% sucrose group were found (146.3±29.3 µm vs 191.0±29.2 µm, p<0.001). Considering the sweetness of isomaltulose is close to half of that of sucrose, it was noted that the mean depth of lesions in the 20% isomaltulose group was smaller than that of the lesions in the 10% sucrose group (p<0.001).
Conclusions: The in-vitro cariogenicity of isomaltulose is much lower than that of sucrose.
Methods: Sixty-four tooth blocks with size of 2.0x2.0x4.0 mm were prepared with 1.0x1.0 mm windows on enamel and dentine by applying acid resistant nail varnish on other surfaces. The blocks were randomly allocated into four groups, 16 each, and placed in media containing (1) 10% isomaltulose, (2) 20% isomaltulose, (3) 10% sucrose, or (4) 20% sucrose, in brain-heart Infusion (BHI) broth. Four cariogenic oral bacteria, Streptococcus mutans, Streptococcus sobrinus, Lactobacillus acidophilus and Lactobacillus rhamnosus, were added to the media to allow biofilm formation and creation of artificial caries lesions in the tooth blocks. These were then incubated anaerobically at 37°C. After every 24 hours, the medium was refreshed manually. Depth of artificial caries lesions created after seven days was measured by micro-CT scanning.
Results: Artificial caries lesions in enamel were found in most of the tooth blocks in the sucrose groups (12 in 10% group and 10 in 20% group), but in only 2 blocks in each the isomaltulose groups (p<0.001). Nearly all of the tooth blocks developed caries lesion in dentine. The mean depth of the lesions in dentine in the 10% isomaltulose group was smaller than that of the 10% sucrose group (149.0±36.0 µm vs. 216.6±26.7 µm, p<0.001). Shallower lesions in the 20% isomaltulose group than in the 20% sucrose group were found (146.3±29.3 µm vs 191.0±29.2 µm, p<0.001). Considering the sweetness of isomaltulose is close to half of that of sucrose, it was noted that the mean depth of lesions in the 20% isomaltulose group was smaller than that of the lesions in the 10% sucrose group (p<0.001).
Conclusions: The in-vitro cariogenicity of isomaltulose is much lower than that of sucrose.