IADR Abstract Archives
Antioxidant Potency of Golden Sea Cucumber Extract on Periodontitis Treatment
Objectives: Oxidative stress has the important role in tissue destruction of periodontitis. Catalase enzyme and heat shock protein (Hsp)-70 are involved in host response to periodontal disease. Golden sea cucumber (Sticophus hermanii) extract has been known to have antioxidant components. Adjuctive treatment with antioxidant agent could be considered to attain the better result in periodontal therapy. The aim of this study was to examine the antioxidant potency of Golden Sea Cucumber extract to periodontitis by measuring the catalase activity in salivary submandibular glands and the expression of Hsp-70 in periodontal tissue of Wistar rat
Methods: The study is an experimental laboratories research with post test only control group design. Twenty four male wistar rats were divided into 4 groups, each consisted of 6 rats. Control groups were given 0.2% CMC Na, were normal group and periodontitis group. Periodontitis were induced by Porphyromonas gingivalis ATCC 33277. Treatment groups were periodontitis rats which given Golden Sea Cucumber extract per oral 135mg/ kg BW once daily (PO group) and applied 0.1ml of 3% extract gel topically on gingival sulcus (TO group) once daily, in 14 consecutive days. The level of catalase were examined from salivary submandibular glands, measured its absorbance by spectrophotometer in 240 nm. Hsp-70 expression were examined from immunohistochemistry stain of periodontal tissue. Data were analyzed by Anova and Mann-Whitney test.
Results: Catalase activity were decreased in periodontitis induced by P.gingivalis while Hsp-70 expression were increased (p<0.05). Sticophus hermanii extract peroral and topical increased the catalase activity (p>0.05) and reduced the Hsp-70 expression (p<0.05).
Conclusions: Golden Sea Cucumber have antioxidant potency in reducing Hsp-70 expression.
Methods: The study is an experimental laboratories research with post test only control group design. Twenty four male wistar rats were divided into 4 groups, each consisted of 6 rats. Control groups were given 0.2% CMC Na, were normal group and periodontitis group. Periodontitis were induced by Porphyromonas gingivalis ATCC 33277. Treatment groups were periodontitis rats which given Golden Sea Cucumber extract per oral 135mg/ kg BW once daily (PO group) and applied 0.1ml of 3% extract gel topically on gingival sulcus (TO group) once daily, in 14 consecutive days. The level of catalase were examined from salivary submandibular glands, measured its absorbance by spectrophotometer in 240 nm. Hsp-70 expression were examined from immunohistochemistry stain of periodontal tissue. Data were analyzed by Anova and Mann-Whitney test.
Results: Catalase activity were decreased in periodontitis induced by P.gingivalis while Hsp-70 expression were increased (p<0.05). Sticophus hermanii extract peroral and topical increased the catalase activity (p>0.05) and reduced the Hsp-70 expression (p<0.05).
Conclusions: Golden Sea Cucumber have antioxidant potency in reducing Hsp-70 expression.