IADR Abstract Archives
Distributions Of Synergistetes Bacteria Within Diseased and Asymptomatic Periodontal Niches
Objectives: To survey the diversity and distributions of Synergistetes taxa, within diseased and asymptomatic periodontal and peri-implant sites, within a cohort of subjects with both periodontitis and peri-implantitis lesions.
Methods: Four single-site samples of subgingival/submucosal plaque were collected from Chinese subjects (n=18; 9 male/9 female), recruited with informed consent (cohort previously described by Zhuang et al., Clinical Oral Implants Research 2014, doi: 10.1111/clr.12508). Plaque was sampled (using paper points) from one healthy gingival site (HT); one periodontitis site (PPD≥4mm; DT); one healthy peri-implant site (HI); and one peri-implantitis site with PPD≥5mm, presence of BOP, and radiographic bone loss (DI). PCR primer sets were used to selectively PCR-amplify ca. 650 bp regions of the 16S rRNA gene from oral cluster A and B Synergistetes, respectively, from DNA purified from each sample (n=72). Plasmid clone libraries of PCR amplicons were constructed, and ca. 50-60 clones were sequenced for each positive sample. Sequence data was analyzed using various bioinformatic, phylogenetic and statistical approaches
Results: 21/72 single-site samples (from 12/18 subjects) yielded Synergistetes 16S rRNA PCR products: HT (n=7/18), DT (n=6/18), HI (n=2/18), DI (n=6/18). Clone libraries were successfully constructed for these 21 samples, yielding a total of 1,338 quality-filtered 16S rRNA sequences. Sequences were assigned to Synergistetes operational taxonomic units (OTUs, using 98.5% identity cut-off). 20 oral cluster A OTUs and 1 oral cluster B OTU (OTU-004=Pyramidobacter piscolens) were identified. Three Synergistetes OTUs predominated, including Fretibacterium fastidiosum (OTU-003). Neither the prevalence nor clonal abundances of Synergistetes exhibited significant differences between the 4 clinical sites. The numbers of F. fastidiosum clones were positively correlated to gingival index, BOP and levels of bone resorption
Conclusions: Diverse Synergistetes taxa were distributed across diseased and asymptomatic periodontal and peri-implant sites, in subjects with both periodontitis and peri-implantitis. F. fastidiosum levels were correlated with disease-related clinical parameters
Methods: Four single-site samples of subgingival/submucosal plaque were collected from Chinese subjects (n=18; 9 male/9 female), recruited with informed consent (cohort previously described by Zhuang et al., Clinical Oral Implants Research 2014, doi: 10.1111/clr.12508). Plaque was sampled (using paper points) from one healthy gingival site (HT); one periodontitis site (PPD≥4mm; DT); one healthy peri-implant site (HI); and one peri-implantitis site with PPD≥5mm, presence of BOP, and radiographic bone loss (DI). PCR primer sets were used to selectively PCR-amplify ca. 650 bp regions of the 16S rRNA gene from oral cluster A and B Synergistetes, respectively, from DNA purified from each sample (n=72). Plasmid clone libraries of PCR amplicons were constructed, and ca. 50-60 clones were sequenced for each positive sample. Sequence data was analyzed using various bioinformatic, phylogenetic and statistical approaches
Results: 21/72 single-site samples (from 12/18 subjects) yielded Synergistetes 16S rRNA PCR products: HT (n=7/18), DT (n=6/18), HI (n=2/18), DI (n=6/18). Clone libraries were successfully constructed for these 21 samples, yielding a total of 1,338 quality-filtered 16S rRNA sequences. Sequences were assigned to Synergistetes operational taxonomic units (OTUs, using 98.5% identity cut-off). 20 oral cluster A OTUs and 1 oral cluster B OTU (OTU-004=Pyramidobacter piscolens) were identified. Three Synergistetes OTUs predominated, including Fretibacterium fastidiosum (OTU-003). Neither the prevalence nor clonal abundances of Synergistetes exhibited significant differences between the 4 clinical sites. The numbers of F. fastidiosum clones were positively correlated to gingival index, BOP and levels of bone resorption
Conclusions: Diverse Synergistetes taxa were distributed across diseased and asymptomatic periodontal and peri-implant sites, in subjects with both periodontitis and peri-implantitis. F. fastidiosum levels were correlated with disease-related clinical parameters