IADR Abstract Archives
Identification of Periodontal Microbial in Obese With Chronic Periodontitis
Objectives: A positive association between obesity and chronic periodontitis (CP) has been documented. The aim of the study is to quantify and compare the frequency of detection of periodontal pathogens; P. gingivalis, T. forsythia, P. intermedia and A. actinomycetamcomitans in obese and non-obese participants with CP.
Methods: This was a cross sectional study with 52 participants who were diagnosed with moderate to advanced CP, divided into obese (n=28) and non-obese (n=24) groups. Obese is defined as individuals with BMI≥27.5 kg/m2. CP is defined as those with at least ≥2 interproximal sites with CAL ≥3 mm, and ≥2 interproximal sites with PPD ≥4 mm (not on same tooth) or one site with PPD ≥5 mm. The inclusion criteria included those who were ≥30 years old and had at least 12 teeth. Those who had periodontal treatment, antibiotic therapy and systemic anti-inflammatory drugs in the last 4 months were excluded. For each patient, sub-gingival plaque sampling was carried out from the deepest pockets in each quadrant using curettes and pooled. Identification and quantification of periodontal microbial was performed using real-time Polymerase chain reaction (qPCR).
Results: P. gingivalis, T. forsythia and P. intermedia were identified in 93-100% participants, regardless group. Lower frequency of detection of A. actinomycetamcomitans was observed in both groups. However, the obese group (25%) had much lower frequency of detection than the non-obese group (54.2%). A higher mean count for P. gingivalis, T. forsythia and P. intermedia was observed in the non-obese group compared to the obese group (p>0.05). In contrast, mean count for A. actinomycetamcomitans was higher in the obese group (p>0.05).
Conclusions: Frequency of detection of P. gingivalis, T. forsythia and P. intermedia was high for both groups. There was a higher trend of detection of A. actinomycetamcomitans in the non-obese CP group.
Methods: This was a cross sectional study with 52 participants who were diagnosed with moderate to advanced CP, divided into obese (n=28) and non-obese (n=24) groups. Obese is defined as individuals with BMI≥27.5 kg/m2. CP is defined as those with at least ≥2 interproximal sites with CAL ≥3 mm, and ≥2 interproximal sites with PPD ≥4 mm (not on same tooth) or one site with PPD ≥5 mm. The inclusion criteria included those who were ≥30 years old and had at least 12 teeth. Those who had periodontal treatment, antibiotic therapy and systemic anti-inflammatory drugs in the last 4 months were excluded. For each patient, sub-gingival plaque sampling was carried out from the deepest pockets in each quadrant using curettes and pooled. Identification and quantification of periodontal microbial was performed using real-time Polymerase chain reaction (qPCR).
Results: P. gingivalis, T. forsythia and P. intermedia were identified in 93-100% participants, regardless group. Lower frequency of detection of A. actinomycetamcomitans was observed in both groups. However, the obese group (25%) had much lower frequency of detection than the non-obese group (54.2%). A higher mean count for P. gingivalis, T. forsythia and P. intermedia was observed in the non-obese group compared to the obese group (p>0.05). In contrast, mean count for A. actinomycetamcomitans was higher in the obese group (p>0.05).
Conclusions: Frequency of detection of P. gingivalis, T. forsythia and P. intermedia was high for both groups. There was a higher trend of detection of A. actinomycetamcomitans in the non-obese CP group.