IADR Abstract Archives
Acute Toxicity Tests of Cobalt-Chromium as Medical Devices
Objectives: A wide range of metals have been developed for medical uses. The cytotoxicity test is an initial part in identifying the harmful effects of a developed material. Cobalt-chromium were the early alloys used in medical devices and till today they are still very popular. Cobalt is responsible for the tarnish and corrosion resistance and chromium increases the strength of the material. The objective of the research is to study the acute toxicity of CoCr for medical devices (in vitro and in vivo levels).
Methods: In vitro test was performed using fibroblast (Vero cell line, LPPT UGM). Cells were treated for 24 hours with three different concentrations (100,1000,10000 µg/mL) of CoCr (Otocompo, Sweden). The cytotoxicity test (number of viability cells) was measured with the MTT assay. In addition, local implantation was performed in 20 rabbits (divided into treatment and control groups). Discs with the diameter of 10mm and 1mm thick, were subcutaneously implanted into the treatment group. Toxic symptom was recorded for the number of lymphocytes, 14th day after the implantation.
Results: The number of viability cells showed an average of 84.92±0.33 (100µg/mL); 84.77±1.59 (1000µg/mL); 83.65±0.63 (10000µg/mL). Statistical analysis showed there was no significant difference (p>0.05) in the viability of the cells among the three concentrations of CoCr. As for the implantation test, the average of the number of lymphocytes 14th day after the treatment were 1.2±1.03 (control) and 1.2±1.22 (treatment). Statisticaly there was no significant difference (p>0.05) in the toxic symptom between the control and treatment groups.
Conclusions: Alloy of CoCr showed no indication in eliciting acute toxicity (in vitro and in vivo levels).
Methods: In vitro test was performed using fibroblast (Vero cell line, LPPT UGM). Cells were treated for 24 hours with three different concentrations (100,1000,10000 µg/mL) of CoCr (Otocompo, Sweden). The cytotoxicity test (number of viability cells) was measured with the MTT assay. In addition, local implantation was performed in 20 rabbits (divided into treatment and control groups). Discs with the diameter of 10mm and 1mm thick, were subcutaneously implanted into the treatment group. Toxic symptom was recorded for the number of lymphocytes, 14th day after the implantation.
Results: The number of viability cells showed an average of 84.92±0.33 (100µg/mL); 84.77±1.59 (1000µg/mL); 83.65±0.63 (10000µg/mL). Statistical analysis showed there was no significant difference (p>0.05) in the viability of the cells among the three concentrations of CoCr. As for the implantation test, the average of the number of lymphocytes 14th day after the treatment were 1.2±1.03 (control) and 1.2±1.22 (treatment). Statisticaly there was no significant difference (p>0.05) in the toxic symptom between the control and treatment groups.
Conclusions: Alloy of CoCr showed no indication in eliciting acute toxicity (in vitro and in vivo levels).