IADR Abstract Archives
The Effect of Nano Chitosan on Odontoblast Cell Proliferation
Objectives: Caries with opened dental pulp can cause inflammation and risk of become in to pulpitis. Direct pulp capping is a technique widely used to cover directly over the open dental pulp. Nano chitosan (100nm) can perform stimulation (excited) in the cell system more effectively because they can pass through cell membranes in organisms more easily and interact with biological systems, so it can be used as a good material for direct pulp capping. The aim of this study is to prove the effect of nano chitosan in enhancing odontoblasts cell proliferation in forming of reparative dentine that can be used as direct pulp capping alternative in open dental pulp in the future.
Methods: Twenty four samples of wistar rats were used and randomly divided into four groups: G1(negative control)-applied with eugenol; G2-applied with MTA; G3-applied with chitosan; G4-applied with nano chitosan. Tooth preparation was performed on the occlusal surface of rat molar teeth until the pulp and then the appropriate liner was applied. Afterwards GIC (Fuji IX) was used as a restoration material. On the 30th day, the rats were sacrified and the jaws were excised. The odontoblast cell proliferation in forming of reparative dentin under the lining was observed using a camera microscope. Data were analyzed with ANOVA and Tukey’s HSD test.
Results: One way ANOVA test showed a significant difference in the mean odontoblast cell proliferation in forming of reparative dentine among the four groups (p<0.001). Tukey's test showed that the mean odontoblast cell proliferation in forming of reparative dentine after molar pulp capping with nano chitosan was the highest (mean=940.0) compared with the other group of eugenol (mean=646.7, p<0.001), MTA (mean=760.0, p<0.001), and chitosan (mean=790.0, p<0.001). However, no statistical difference was found for odontoblast cell proliferation in forming of reparative dentine after molar pulp capping with chitosan compared with MTA (p=0.640).
Conclusions: Nano chitosan can increase odontoblast cell proliferation in forming of reparative dentine so tooth’s vitality will be more protected.
Methods: Twenty four samples of wistar rats were used and randomly divided into four groups: G1(negative control)-applied with eugenol; G2-applied with MTA; G3-applied with chitosan; G4-applied with nano chitosan. Tooth preparation was performed on the occlusal surface of rat molar teeth until the pulp and then the appropriate liner was applied. Afterwards GIC (Fuji IX) was used as a restoration material. On the 30th day, the rats were sacrified and the jaws were excised. The odontoblast cell proliferation in forming of reparative dentin under the lining was observed using a camera microscope. Data were analyzed with ANOVA and Tukey’s HSD test.
Results: One way ANOVA test showed a significant difference in the mean odontoblast cell proliferation in forming of reparative dentine among the four groups (p<0.001). Tukey's test showed that the mean odontoblast cell proliferation in forming of reparative dentine after molar pulp capping with nano chitosan was the highest (mean=940.0) compared with the other group of eugenol (mean=646.7, p<0.001), MTA (mean=760.0, p<0.001), and chitosan (mean=790.0, p<0.001). However, no statistical difference was found for odontoblast cell proliferation in forming of reparative dentine after molar pulp capping with chitosan compared with MTA (p=0.640).
Conclusions: Nano chitosan can increase odontoblast cell proliferation in forming of reparative dentine so tooth’s vitality will be more protected.