IADR Abstract Archives
Horsetail Extract For Regeneration Of Condylar Cartilage With TMJ Osteoarthritis
Objectives: TMJ Osteosrthritis (TMJOA) is a unilateral, degenerative disease characterized by breakdown of articular cartilage, architectural changes in bone and degeneration of synovial tissue. The purpose of the study is to determine effectiveness of horsetail extract in regenerating condylar cartilage.
Methods: Sixteen female New Zealand rabbits divided into three groups: positive, negative and experimental; with induced osteoarthritis on their right TMJ by intra-articular injection of 5mg Monosodium Iodoacetate dissolved in 1ul saline. One rabbit was sacrificed 3 weeks post-injection to verify TMJOA. Once verified histologically , treatment began. 1.2g/ml/kg horsetail extract taken orally for experimental group, with the same dose of hyaluronic acid for positive group. Tissue specimens were collected 2 weeks post-treatment and assessed histologically. P-value was determined using Analysis of Variance and Bonferroni’s correction for pair-wise comparisons.
Results: Negative control: "moderate" inflammation and "very mild" healing. Positive control: "very mild" inflammation and "good" healing. Experimental group: "very mild" to "no inflammation" and "good" to "excellent" healing. Regarding inflammation, there was significant difference (P=0.009<0.05) between negative control (mean=3.00±0.00) and positive control (mean=1.5 ± 0.58) indicating lesser inflammation in positive control; significant difference (P=0.001<0.05) between negative control and experimental group (mean=1.00±0.71) indicating less inflammation in experimental group; and no significant difference (P=0.610>0.05) between positive control and experimental group showing identical levels of inflammation. Regarding cartilage regeneration, significant difference (P=0.000<0.05) was found between negative control (mean=1.00±-0.00) and positive control (mean=2.38±0.25) indicating greater healing in positive control; significant difference (P=0.000<0.05) between negative control and experimental group (mean=2.40±0.42) indicating greater healing in experimental group, and no significant difference (P=1.000>0.05) between positive control and experimental group showing cartilage healing at identical levels.
Conclusions: Horsetail extract is effective in regenerating condylar cartilage with TMJOA.
Methods: Sixteen female New Zealand rabbits divided into three groups: positive, negative and experimental; with induced osteoarthritis on their right TMJ by intra-articular injection of 5mg Monosodium Iodoacetate dissolved in 1ul saline. One rabbit was sacrificed 3 weeks post-injection to verify TMJOA. Once verified histologically , treatment began. 1.2g/ml/kg horsetail extract taken orally for experimental group, with the same dose of hyaluronic acid for positive group. Tissue specimens were collected 2 weeks post-treatment and assessed histologically. P-value was determined using Analysis of Variance and Bonferroni’s correction for pair-wise comparisons.
Results: Negative control: "moderate" inflammation and "very mild" healing. Positive control: "very mild" inflammation and "good" healing. Experimental group: "very mild" to "no inflammation" and "good" to "excellent" healing. Regarding inflammation, there was significant difference (P=0.009<0.05) between negative control (mean=3.00±0.00) and positive control (mean=1.5 ± 0.58) indicating lesser inflammation in positive control; significant difference (P=0.001<0.05) between negative control and experimental group (mean=1.00±0.71) indicating less inflammation in experimental group; and no significant difference (P=0.610>0.05) between positive control and experimental group showing identical levels of inflammation. Regarding cartilage regeneration, significant difference (P=0.000<0.05) was found between negative control (mean=1.00±-0.00) and positive control (mean=2.38±0.25) indicating greater healing in positive control; significant difference (P=0.000<0.05) between negative control and experimental group (mean=2.40±0.42) indicating greater healing in experimental group, and no significant difference (P=1.000>0.05) between positive control and experimental group showing cartilage healing at identical levels.
Conclusions: Horsetail extract is effective in regenerating condylar cartilage with TMJOA.