IADR Abstract Archives
Antimicrobial Biosurfactants – Potential Green Detergents in Oral Care Products
Objectives: Oral cavity is exposed to thousands of microorganisms daily. A healthy oral cavity is of utmost importance to the overall health of any individual. Numerous oral care products have flooded the market. Most of these possess detergents which enable foaming and emulsification. Unfortunately these become persistent pollutants in the environment. The present study attempts to screen biodegradable yet stable molecules which can be a substitute to detergents. Biosurfactant, amphipathic molecule produced by bacteria and fungi possess detergent like properties, could be an alternative. The study aims to determine the surfactant properties, emulsification efficiency and antimicrobial activity of compound purified from soil bacteria.
Methods: Soil microorganisms C1 and C3 (Madhumathi et al., 2010) were screened for the production of biosurfactants by Drop collapse Assay (DCA). Reduction of surface tension was determined by capillary rise method. Emulsification Index (EI) was measured to assess the stability of biosurfactant. Determination of Antimicrobial Property against organisms isolated from an oral swab was carried out by Kirby Baur method.
Results: All the data presented are mean values of 3determinations and statistically compared using ANOVA (P< 0.05). The organisms screened were positive for biosurfactant production. The reduction of surface tension of water by the biosurfactant (from 72.75mN/m to 44mN/m) was comparable with that of synthetic surfactant SDS (from 72.75mN/m to 46.75mN/m). The biosurfactants from C1 and C3 retained upto 88% EI by 7days, which was 10% higher than that of SDS. Biosurfactants were antimicrobial against Streptococcus and Lactobacillus sp. isolated from oral swab.
Conclusions: The Biosurfactants under study showed a very good antimicrobial nature and exhibited all the detergent like properties. This indicates that biosurfactants may be the potential candidates as supplements in the oral care products.
Key words: Oral cavity, Oral Care, Antimicrobial, Streptococcus, Lactobacillus.
Methods: Soil microorganisms C1 and C3 (Madhumathi et al., 2010) were screened for the production of biosurfactants by Drop collapse Assay (DCA). Reduction of surface tension was determined by capillary rise method. Emulsification Index (EI) was measured to assess the stability of biosurfactant. Determination of Antimicrobial Property against organisms isolated from an oral swab was carried out by Kirby Baur method.
Results: All the data presented are mean values of 3determinations and statistically compared using ANOVA (P< 0.05). The organisms screened were positive for biosurfactant production. The reduction of surface tension of water by the biosurfactant (from 72.75mN/m to 44mN/m) was comparable with that of synthetic surfactant SDS (from 72.75mN/m to 46.75mN/m). The biosurfactants from C1 and C3 retained upto 88% EI by 7days, which was 10% higher than that of SDS. Biosurfactants were antimicrobial against Streptococcus and Lactobacillus sp. isolated from oral swab.
Conclusions: The Biosurfactants under study showed a very good antimicrobial nature and exhibited all the detergent like properties. This indicates that biosurfactants may be the potential candidates as supplements in the oral care products.
Key words: Oral cavity, Oral Care, Antimicrobial, Streptococcus, Lactobacillus.