IADR Abstract Archives
Bisphenol-A Release and Genotoxicity Differences of Three Branded Adhesives Materials
Objectives: Fixed retainer usually bonded with resin based composites which contains bisphenol-A (BPA) in Bis-GMA and Bis-EMA form. BPA is a synthetic chemical with known disruptive endocrine and weak estrogenic properties. The degradation of these materials can leach BPA into the oral environment., which could be a clinical concern and could be exacerbated by temperature changes, mechanical wear, changes in pH, and bacterial or salivary enzymatic action. BPA release and genotoxic activity of adhesives used in bonding fixed retainer has not been studied yet. The objective of the study was to determine BPA release and genotoxicity differences between three adhesives, and to determine the relationshipo between BPA release and genotoxicity.
Methods: Three branded adhesives materials were polymerized in mold and immersed in pH 7 and 4 artificial saliva from 24 to 720 hours. The artificial saliva was tested with spectrophotometry test to see BPA release at 24, 240, 480, and 720 hours, then freeze dried to get solid extract. Combination of the extract and lymphocyte culture (male and female) then tested with in vitro cytokinesis-block micronucleus (MN) assay to see genotoxicity level of three adhesives at 24, 240, 480, and 720 hours as well.
Results: The highest BPA release occured at 720 hours by Tetric N-Flow: 0.013μg/L; Filtek Z350XT: 0.11μg/L; Flowtain LV: 0.036μg/L respectively. There was a statistically significant difference between BPA release with time (F = 505.98; p<0.01) and brands (F = 147.65; p<0.01). Time and BPA release interaction also showed a statistically significant difference (F=13.35; p<0.01). Genotoxicity can be seen at 720 hours on Flowtain LV sample (MN frequency: male: 0.044; female: 0.053).
Conclusions: The number of BPA release of all brands was increasing from 24 to 720 hours and did not influence by brands. Genotoxicity can be seen from one of the adhesive brand at 720 hours. There was correlation between BPA leaching and micronucleus frequency.
Methods: Three branded adhesives materials were polymerized in mold and immersed in pH 7 and 4 artificial saliva from 24 to 720 hours. The artificial saliva was tested with spectrophotometry test to see BPA release at 24, 240, 480, and 720 hours, then freeze dried to get solid extract. Combination of the extract and lymphocyte culture (male and female) then tested with in vitro cytokinesis-block micronucleus (MN) assay to see genotoxicity level of three adhesives at 24, 240, 480, and 720 hours as well.
Results: The highest BPA release occured at 720 hours by Tetric N-Flow: 0.013μg/L; Filtek Z350XT: 0.11μg/L; Flowtain LV: 0.036μg/L respectively. There was a statistically significant difference between BPA release with time (F = 505.98; p<0.01) and brands (F = 147.65; p<0.01). Time and BPA release interaction also showed a statistically significant difference (F=13.35; p<0.01). Genotoxicity can be seen at 720 hours on Flowtain LV sample (MN frequency: male: 0.044; female: 0.053).
Conclusions: The number of BPA release of all brands was increasing from 24 to 720 hours and did not influence by brands. Genotoxicity can be seen from one of the adhesive brand at 720 hours. There was correlation between BPA leaching and micronucleus frequency.