IADR Abstract Archives
Anti-plaque Effect of Green Tea and Salvadora persica
Objectives: This study aimed to investigate the synergistic anti-plaque effect of a combination of green tea (Gt) and Salvadora persica (Sp) aqueous extracts against biofilm of Streptococcus mitis, Streptococcus sanguinis and Actinomyces viscosus.
Methods: Two-fold serial micro-dilution method was used to measure minimal inhibitory concentration (MIC) of aqueous extracts of Gt, Sp and their combinations. Adsorption to hexadecane was used to determine the cell surface hydrophobicity (CSH) of bacterial cells. Glass beads coated with saliva were used as experimental pellicles in adhesion of early colonizing bacteria to hard tissue surfaces.
Results: Gt aqueous extracts (MIC=0.98mg/ml) exhibited better anti-plaque effect than Sp aqueous extracts (MIC=15.63mg/ml). The combination of 0.25mg/ml Gt extracts and 7.5mg/ml Sp extracts, which are equivalent to ¼ and ½ of MIC values of Gt and Sp extracts respectively, showed synergistic anti-plaque properties. This combination was found to significantly reduced CSH (from 62% to 50%, p<0.05 Mann-Whitney U) and adherence (mean log10 from 3.0971CFU/ml to 2.631CFU/ml, p<0.003 Mann-Whitney U) to experimental pellicles.
Conclusions: Combination between Gt and Sp aqueous extracts exhibited synergistic anti-plaque activity, and could be used as useful active agent for the development of oral health products.
Methods: Two-fold serial micro-dilution method was used to measure minimal inhibitory concentration (MIC) of aqueous extracts of Gt, Sp and their combinations. Adsorption to hexadecane was used to determine the cell surface hydrophobicity (CSH) of bacterial cells. Glass beads coated with saliva were used as experimental pellicles in adhesion of early colonizing bacteria to hard tissue surfaces.
Results: Gt aqueous extracts (MIC=0.98mg/ml) exhibited better anti-plaque effect than Sp aqueous extracts (MIC=15.63mg/ml). The combination of 0.25mg/ml Gt extracts and 7.5mg/ml Sp extracts, which are equivalent to ¼ and ½ of MIC values of Gt and Sp extracts respectively, showed synergistic anti-plaque properties. This combination was found to significantly reduced CSH (from 62% to 50%, p<0.05 Mann-Whitney U) and adherence (mean log10 from 3.0971CFU/ml to 2.631CFU/ml, p<0.003 Mann-Whitney U) to experimental pellicles.
Conclusions: Combination between Gt and Sp aqueous extracts exhibited synergistic anti-plaque activity, and could be used as useful active agent for the development of oral health products.