IADR Abstract Archives
Differential Effects of Moringa oleifera Aqueous Leaf Extracts on Osteoblasts
Objectives: Moringa Oleifera has many ingredients that are beneficial to bone tissues. Although several studies demonstrated the positive effects of Moringa Oleifera extracts on fracture healing, no studies reported the direct effects of Moringa Oleifera aqueous extracts on osteoblasts. The aims of this study were to investigate the cytotoxic effect of Moringa Oleifera aqueous leaf extracts and their effects on proliferation and differentiation of osteoblasts.
Methods: The aqueous leaf extracts of Moringa Oleifera were prepared by decoction or maceration at room temperature. MC3T3-E1 osteoblast-like cells were cultured in the presence of Moringa Oleifera aqueous leaf extracts from 0 to 800 µg/ml for 48 hr and analyzed by MTT assay. Osteoblast differentiation after 7 and 15 days of culture was analyzed by alkaline phosphatase enzyme activity assay and mineralization of osteoblast culture was analyzed by Alizarin Red assay. Statistical analyses were performed using ANOVA with multiple comparison tests.
Results: The results showed that Moringa Oleifera aqueous leaf extract by decoction had significantly less cytotoxicity than aqueous extract by maceration at room temperature (p<0.05). Treatment of aqueous leaf extract by maceration at 125 µg/ml significantly increased osteoblast cell proliferation (p<0.05) while no proliferative effect of aqueous extract by decoction was observed. Moringa Oleifera aqueous leaf extracts by both methods, however, had no effects on alkaline phosphatase enzyme activities. Finally, mineralization assay showed that calcium deposition or mineralization of osteoblasts was significantly increased after treatment with aqueous leaf extract by decoction at 50-125 µg/ml (p<0.05), while no changes were observed after treatment with aqueous extract by maceration at room temperature.
Conclusions: We demonstrated that Moringa Oleifera aqueous leaf extract by decoction was less toxic to osteoblast cells and enhanced mineralization by osteoblasts when compared to Moringa Oleifera aqueous extract by maceration at room temperature.
Methods: The aqueous leaf extracts of Moringa Oleifera were prepared by decoction or maceration at room temperature. MC3T3-E1 osteoblast-like cells were cultured in the presence of Moringa Oleifera aqueous leaf extracts from 0 to 800 µg/ml for 48 hr and analyzed by MTT assay. Osteoblast differentiation after 7 and 15 days of culture was analyzed by alkaline phosphatase enzyme activity assay and mineralization of osteoblast culture was analyzed by Alizarin Red assay. Statistical analyses were performed using ANOVA with multiple comparison tests.
Results: The results showed that Moringa Oleifera aqueous leaf extract by decoction had significantly less cytotoxicity than aqueous extract by maceration at room temperature (p<0.05). Treatment of aqueous leaf extract by maceration at 125 µg/ml significantly increased osteoblast cell proliferation (p<0.05) while no proliferative effect of aqueous extract by decoction was observed. Moringa Oleifera aqueous leaf extracts by both methods, however, had no effects on alkaline phosphatase enzyme activities. Finally, mineralization assay showed that calcium deposition or mineralization of osteoblasts was significantly increased after treatment with aqueous leaf extract by decoction at 50-125 µg/ml (p<0.05), while no changes were observed after treatment with aqueous extract by maceration at room temperature.
Conclusions: We demonstrated that Moringa Oleifera aqueous leaf extract by decoction was less toxic to osteoblast cells and enhanced mineralization by osteoblasts when compared to Moringa Oleifera aqueous extract by maceration at room temperature.