IADR Abstract Archives

Inhibition of Oral Organisms by Ora-Salts

Gingivitis, mouth ulcers, sore throats, puffy bleeding gums and bad breath not only cause a lot of discomfort in patients, but also have one common reality, namely they are all associated with microorganisms.  Ora-Salts is an all-natural product that can be used for the treatment of the mentioned ailments.  Objective:  To determine the inhibition–potential of Ora-Salts for caries organisms, the major periodontal pathogens and Candida albicansMethods:  Inhibition of growth was done by the tube dilution technique (conditions standardized and test agent present throughout entire incubation period).  Increasing concentrations of Ora-Salts (0–5% m/v) was prepared in CASO broth, with the nutritional composition in all tubes exactly the same.  Series were inoculated with standardized suspensions of Streptococcus mutans, Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans, Prevotella intermedia, Tannerella forsythensis, Eikenella corrodens and Candida albicans.  All tubes were incubated at 37oC for 48h and in an anaerobic atmosphere where necessary created with Anaerocult A (Merck).  Control tubes containing media only were included.  After 48 hours growth in the different tubes in terms of turbidity was measured with a spectrophotometer at a wavelength of 540nm.  All growth experiments were repeated in triplicate.  Analysis of results was done by ANOVA (Statistics V8 software).  Results:  No inhibition of growth of any of the test organisms was observed.  Growth of organisms actually seemed to be stimulated by the presence of the Ora-Salts as mean growth of the organisms at the 5% concentration was higher than mean growth in the control tubes (see Table).

 

<>Organism

Growth in Control Tube (Absorbance at 540nm)

Growth in 5% Ora-Salts (Absorbance at 540nm)

S. mutans

0.973

2.101

P. gingivalis

1.834

1.833

A. actinomycetemcomitans

1.240

1.269

P. intermedia

0.730

1.263

T. forsythensis

0.722

1.746

E. corrodens

0.760

1.505

C. albicans

0.704

1.091

Conclusion:  Ora-Salts had no antimicrobial activity against the tested oral organisms.


Division: South African Division
Meeting: 2006 South African Division (Midrand, South Africa)
Location: Midrand, South Africa
Year: 2006
Final Presentation ID:
Abstract Category|Abstract Category(s): Scientific Groups
Authors
  • Molobela, Itumeleng P.  ( University of Pretoria, Pretoria, N/A, South Africa )
  • Botha, Stephanus J.  ( University of Pretoria, Pretoria, N/A, South Africa )
  • SESSION INFORMATION
    Oral Session
    Microbiology/Immunology/Infection Control