IADR Abstract Archives

Cytotoxicity and Antimicrobial Effect of Green Synthesized Silver Nanoparticles

Objectives: The objective of this study was to test the cytotoxicity and antimicrobial effect of silver nanoparticles (AgNPs) manufactured by means of green synthesis using plant extract against microorganisms associated with oral biofilm.
Methods: Two types of AgNPs (4g/1g) were synthesized from a plant extract. These AgNPs were characterized via UV-Vis spectral analysis, DLS, FTIR, and TEM. Additionally, the stability of both AgNPs was tested in artificial media representing the condition on the oral cavity. Subsequently, their antimicrobial activity was tested against S.sanguinis, S.mutans, L.acidophilus, and C.albicans using disc diffusion method. Their minimum inhibitory concentrations (MICs) were established. Finally, cytotoxicity was tested on the buccal mucosa fibroblast cells (BMF) using MTT assay.
Results: Both AgNPs were spherical, ranging between 10-20nm in diameter and were more stable in Mullier Hinton Broth compared to the other media tested. 4gAgNPs had relatively comparable zones of inhibition to chlorohexidine, whereas those for 1gAgNPs were negligible. The average MIC90 & MIC50 for 4gAgNPs range between 1.5625-12.5µg/ml, with 25-100µg/ml for 1gAgNPs. The 1gAgNPs had 60% survival at 100µg/ml, with 4gAgNPs above 80%, suggesting lower cytotoxicity in 4g.
Conclusions: 4gAgNPs showed a relatively higher antimicrobial activity against the tested oral microbes, with lesser cytotoxicity than 1g. Thus, 4gAgNPs has potential to influence oral biofilm formation.

2021 South African Division Meeting (Virtual)
Virtual
2021

Cariology Research-Microbiological Studies/Biofilm
  • Ahmed, Omnia  ( UWC , Cape Town , Western Cape , South Africa )
  • Maboza, Ernest  ( UWC , Cape Town , western cape , South Africa )
  • Geerts, Greta  ( University of the Western Cape , Tygerberg , Western Cape Province , South Africa )
  • none
    Poster Session
    Abstracts Accepted