IADR Abstract Archives
Anticariogenic properties of Dodonaea viscosa var. angustifolia flavone stabilized nanoparticles
Objectives: Dental caries is the most important oral infection. It is caused by Streptococcus mutans due to its ability to form biofilm and the production of acids in the oral cavity. Many oral hygiene products containing antimicrobial chemicals are used to control and prevent dental caries. Medicinal plants have been investigated for their ability to prevent dental caries. Dodonaea viscosa var. angustifolia, has been found to have this property. However, beneficial concentrations are difficult to maintain in the oral cavity due to continual saliva flow which can be overcome using nanotechnology. The aim of this study was to investigate the anticariogenic properties of Dodonaea viscosa var. angustifolia (DVA) derived flavone stabilized polymeric nanoparticles.
Methods: Crude extract prepared from DVA leaves was fractionated to produce subfractions and the beneficial subfraction was identified. Polymeric nanoparticles were prepared, stabilized with the DVA subfraction and characterized. Anti-S. mutans and anti-acidogenic properties were determined using well described techniques. The subfraction release profile (substantivity) was determined using dialysis tubing technique. Results of the acid assays were analyzed using the Wilcoxon sum test (Mann-Whitney).
Results: The results showed that subfraction stabilized nanoparticles had anti-S. mutans property with a median MIC value of 3.125 mg/ml. Subinhibitory concentrations of these nanoparticles significantly reduced the acid production in S. mutans (p=<0.05). Polymeric nanoparticles also showed some anti-S. mutans and anti-acidogenic activity. The retention and slow release of the beneficial compound was detected up to 12 hours, reaching 0.1 mg/ml concentration at pH 7.4 after 4 hours and at pH 5.5 after 5 hours.
Conclusions: In conclusion, the DVA flavone containing subfraction stabilized nanoparticles showed anticariogenic activity with improved substantivity. Therefore, it has potential to be used to control dental caries.
Methods: Crude extract prepared from DVA leaves was fractionated to produce subfractions and the beneficial subfraction was identified. Polymeric nanoparticles were prepared, stabilized with the DVA subfraction and characterized. Anti-S. mutans and anti-acidogenic properties were determined using well described techniques. The subfraction release profile (substantivity) was determined using dialysis tubing technique. Results of the acid assays were analyzed using the Wilcoxon sum test (Mann-Whitney).
Results: The results showed that subfraction stabilized nanoparticles had anti-S. mutans property with a median MIC value of 3.125 mg/ml. Subinhibitory concentrations of these nanoparticles significantly reduced the acid production in S. mutans (p=<0.05). Polymeric nanoparticles also showed some anti-S. mutans and anti-acidogenic activity. The retention and slow release of the beneficial compound was detected up to 12 hours, reaching 0.1 mg/ml concentration at pH 7.4 after 4 hours and at pH 5.5 after 5 hours.
Conclusions: In conclusion, the DVA flavone containing subfraction stabilized nanoparticles showed anticariogenic activity with improved substantivity. Therefore, it has potential to be used to control dental caries.