IADR Abstract Archives

Antibacterial properties of a novel chitosan nanoparticle based intra-canal medicament

Objectives: The aim was to create a novel bioactive nanocomposite intra-canal medicament using chitosan nanoparticles (Ch-Np), loaded into Zeolite-Y as a carrier and evaluates its antimicrobial and biological properties against root canal pathogens.
Methods: Ch-Np were synthesized from low molecular weight chitosan (LMW-Ch) using electrospraying. Ch-Np were characterized for their size and surface charge. The antimicrobial efficacy of Ch-Np was evaluated against S. mutans and E. faecalis in a planktonic state using a time-kill study and against biofilm biomass using microtiter plate biofilm assay. The antimicrobial efficacy of Ch-Np when loaded into Zeolite-Y was evaluated using agar diffusion test. Additionally, the effect of tissue inhibitors (dentine powder and serum albumin) on the antibacterial efficacy of Ch-NP-Zeolite nanocomposite was evaluated using a time-kill study. Finally, the cytotoxicity of LMW-Ch, Ch-Np, Zeolite-Y and Ch-Np-Zeolite nanocomposite was evaluated against 3T3 mouse fibroblast cells.
Results: Electrospraying of LMW-Ch produced Ch-Np with average particle size of (430 nm) and a surface charge of (51.7 mV). Ch-Np eradicated S. mutans and E. faecalis in planktonic state within 30 minutes and 1 hour respectively and significant reduction in the biofilm biomass for both pathogens. Ch-Np loaded into Zeolite showed a mean diameter inhibition zone of 9.6 mm for S. mutans and 7.8 mm for E. faecalis. The presence of tissue inhibitors did not affect the antibacterial efficacy of Ch-Np-Zeolite nanocomposite. When tested for cytotoxicity using 3T3 cells none of the tested materials was shown to be cytotoxic.
Conclusions: The novel Ch-Np- Zeolite nanocomposite completely eradicated S. mutans and E. faecalis even in the presence of tissue inhibitors. Furthermore it was shown to be non-cytotoxic.
South African Division Meeting
2018 South African Division Meeting (Gauteng Province, South Africa)
Gauteng Province, South Africa
2018

  • Ibrahim, Amir  ( University of the western cape , Cape Town , Western Cape , South Africa )
  • Moodley, D.s.  ( University of the western cape , Cape Town , Western Cape , South Africa )
  • Petrik, Leslie  ( University of The western Cape , Cape Town , Western Cape , South Africa )
  • Maboza, Ernest  ( University of The western Cape , Cape Town , Western Cape , South Africa )
  • Olivier, Annette  ( University of The western Cape , Cape Town , Western Cape , South Africa )
  • none
    Poster Session
    Abstracts Presented