IADR Abstract Archives
Biomimetic Hydroxyapatite-based Toothpaste on Bacterial Colonization of Enamel and Composite
Objectives: The aim of this study was to evaluate in vitro the effect of toothpaste formulations containing hydroxyapatite nano-particles on enamel and composite early colonization and biofilm formation by mixed plaque flora and by S. mutans using both a microtiter plate model and a MDFR.
Methods: A total of 128 enamel disks obtained from anterior human teeth and 128 disks with the same dimensions were obtained from a nanohybrid resin-based composite (RBC, Clearfil Majesty ES-2, shade A2, Kuraray Europe GmbH, Hattersheim am Main, Germany). Three toothpaste formulations were analyzed both on enamel and composite specimens: A) Toothpaste without active principle; B) Toothpaste containing hydroxyapatite nano-particles (Biorepair® Protezione Totale, Coswell, Italy); C) Toothpaste containing Mg-Ca-Sr biomimetic hydroxyapatite (Biosmalto®, Curaden Healthcare Spa, Italy). A control group (D) has been prepared for comparison. The specimens were placed in the MDFR device and then incubated at 37°C for 24 h in clarified saliva. In parallel, it was decided to carry out additional experiments developing mixed plaque and monospecific Streptococcus mutans biofilms inside microtiter plates, under static conditions.
Results: Enamel surfaces were generally less colonized than the RBC counterparts (p<0.0001). In particular, Group C was significantly less colonized than the control on both surfaces (p<0.0001). No significant differences were found between Group B and the control (p=0.8804 and p=0.6892 for RBC and enamel surfaces, respectively). and between Group A and the control on RBC surfaces (p=0.6855).
Conclusions: The fact that S. mutans has more affinity for RBC surfaces than for enamel is very interesting since it may be related to secondary caries occurrence. It is therefore unexpected but of primary importance the fact that a toothpaste treatment may have an influence on RBC surfaces in addition to the natural ones, since this may allow the prevention of secondary caries in addition to primary caries prevention by a toothpaste treatment.
Methods: A total of 128 enamel disks obtained from anterior human teeth and 128 disks with the same dimensions were obtained from a nanohybrid resin-based composite (RBC, Clearfil Majesty ES-2, shade A2, Kuraray Europe GmbH, Hattersheim am Main, Germany). Three toothpaste formulations were analyzed both on enamel and composite specimens: A) Toothpaste without active principle; B) Toothpaste containing hydroxyapatite nano-particles (Biorepair® Protezione Totale, Coswell, Italy); C) Toothpaste containing Mg-Ca-Sr biomimetic hydroxyapatite (Biosmalto®, Curaden Healthcare Spa, Italy). A control group (D) has been prepared for comparison. The specimens were placed in the MDFR device and then incubated at 37°C for 24 h in clarified saliva. In parallel, it was decided to carry out additional experiments developing mixed plaque and monospecific Streptococcus mutans biofilms inside microtiter plates, under static conditions.
Results: Enamel surfaces were generally less colonized than the RBC counterparts (p<0.0001). In particular, Group C was significantly less colonized than the control on both surfaces (p<0.0001). No significant differences were found between Group B and the control (p=0.8804 and p=0.6892 for RBC and enamel surfaces, respectively). and between Group A and the control on RBC surfaces (p=0.6855).
Conclusions: The fact that S. mutans has more affinity for RBC surfaces than for enamel is very interesting since it may be related to secondary caries occurrence. It is therefore unexpected but of primary importance the fact that a toothpaste treatment may have an influence on RBC surfaces in addition to the natural ones, since this may allow the prevention of secondary caries in addition to primary caries prevention by a toothpaste treatment.
2530660_File000000.jpg
2530660_File000001.jpg