IADR Abstract Archives
Evolutionary Conservation of Human and Murine Oral Mucosa Stem Cells
Objectives: To determine the evolutionary conservation of the transcriptome of human oral mucosa stem cells (hOMSC) and murine oral mucosa stem cells (mOMSC) and compare their potential to support skin wound healing.
Methods: hOMSC and mOMSC cultures were obtained from biopsies of human and murine oral mucosa. mRNA was extracted from the initial biopsies and cultures of hOMSC and mOMSC. Affymetrix GeneChip was used to hybridize cDNAs. The level of gene expression in biopsies vs. cell cultures was determined and compared by ANOVA and validated by qRT-PCR. hOMSC and mOMSC were injected into the dermis at the border of 6 mm circular skin wounds in 16 C57BL/6 mice and tested for their capacity to support wound healing compared to untreated controls.
Results: 16% of the human genome and 9% of the mouse genome were differentially expressed in cell cultures compared to biopsies. 4672 genes were up-regulated at fold change (FC) 2 in the human genome, and 2529 genes in the mouse genome, 1001 genes being common. Down-regulated genes at fold change 2 totaled 2134 genes in the human genome, and 2334 genes in the mouse genome, 445 being common. DAVID and WebGestalt bioinformatics tools revealed that the common pathways of the common differentially expressed genes belong to cell cycle, cell migration, cell attachment, vascular biology, senescence and apoptosis pathways. Both cell types enhanced skin wound healing compared to untreated controls.
Conclusions: The results show basic stem cell biological function similarities between hOMSC and mOMSC, which play crucial roles for preclinical translational studies.
Methods: hOMSC and mOMSC cultures were obtained from biopsies of human and murine oral mucosa. mRNA was extracted from the initial biopsies and cultures of hOMSC and mOMSC. Affymetrix GeneChip was used to hybridize cDNAs. The level of gene expression in biopsies vs. cell cultures was determined and compared by ANOVA and validated by qRT-PCR. hOMSC and mOMSC were injected into the dermis at the border of 6 mm circular skin wounds in 16 C57BL/6 mice and tested for their capacity to support wound healing compared to untreated controls.
Results: 16% of the human genome and 9% of the mouse genome were differentially expressed in cell cultures compared to biopsies. 4672 genes were up-regulated at fold change (FC) 2 in the human genome, and 2529 genes in the mouse genome, 1001 genes being common. Down-regulated genes at fold change 2 totaled 2134 genes in the human genome, and 2334 genes in the mouse genome, 445 being common. DAVID and WebGestalt bioinformatics tools revealed that the common pathways of the common differentially expressed genes belong to cell cycle, cell migration, cell attachment, vascular biology, senescence and apoptosis pathways. Both cell types enhanced skin wound healing compared to untreated controls.
Conclusions: The results show basic stem cell biological function similarities between hOMSC and mOMSC, which play crucial roles for preclinical translational studies.