IADR Abstract Archives

Electrospun Nano Fibers With Controlled Release hVEGF Promote Angiogenesis

Objectives: Angiogenesis is critical in all aspects of tissue regeneration. The aim of this study is to develop an electrospun fibers scaffold that permits controlled release of recombinant human VEGF (rhVEGF) to enhance angiogenesis.
Methods: Scaffolds composed of core-shell fibers were fabricated by electrospinning process. The shell solution was composed of polycarpolactone 8% and polyethylene glycol (PEG) in various concentrations (0.25-3%) to produce pores of different sizes on the shell. The core solution was composed of polyethylene oxide (PEO) 4% and mixed with rhVEGF. SEM was used to characterize the scaffold and measure pore size. The releasing kinetics of rhVEGF was monitored by ELISA. In-vitro biological activity of rhVEGF was determined by trans-well migration assay. In-vivo angiogenesis was evaluated by subcutaneous implantation of the scaffold in a mouse model. Recruitment of cells into the scaffold and angiogenesis was evaluated via confocal microscopy and histomorphometry, after 3 and 14 days respectively.
Results: Core-shell fibers of 6-8 µm diameter was obtained. SEM analysis revealed a positive correlation between PEG concentration and pore size in the shell. Mean pore size of the shell was 503.497±64 nm in 3%PEG and 205±60 nm in 1%PEG. Similarly, changes in PEG concentrations influenced rhVEGF release: the maximum release of rhVEGF from 3%PEG scaffold was 23% within 4h; while 1%PEG released 38% of rhVEGF within 18h. Transwell migration assay demonstrated that 1%PEG scaffold loaded with rhVEGF enhanced endothelial cells (EC) migration by 80 folds compared with negative control (1%PEG scaffold without rhVEGF). In-vivo model: increased recruitment of cells in the test group after 3 days. Likewise, angiogenesis was also significantly higher in the test group after 3 and 14 days (P<0.01)
Conclusions: PEG concentration influenced pore size of the scaffold shell thereby modified rhVEGF release kinetics. Released rhVEGF promoted ECs migration in-vitro and significantly enhanced blood vessel formation.

IADR/PER Congress
2016 IADR/PER Congress (Jerusalem, Israel)
Jerusalem, Israel
2016
0050
Mineralized Tissue
  • Khutaba, Alaa  ( technion , Arraba , Israel ;  rambam health care campus , Haifa , Israel )
  • Zigdon-giladi, Hadar  ( rambam health care campus , Haifa , Israel )
  • Srouji, Samer  ( Galilee Medical Center , Haifa , Israel ;  Galilee Medical Center , Nahyria , Israel )
  • Machtei, Eli  ( Rambam Health Care Campus , Haifa , Israel )
  • Elimelech, Rina  ( rambam health care campus , Haifa , Israel )
  • no conflicts of interest
    Oral Session
    Mineralized Tissue
    Wednesday, 09/21/2016 , 09:00AM - 10:30AM